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首页> 外文期刊>Journal of Virology >Deletion of a 9,000-base-pair segment of the vaccinia virus genome that encodes nonessential polypeptides.
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Deletion of a 9,000-base-pair segment of the vaccinia virus genome that encodes nonessential polypeptides.

机译：删除编码非必要多肽的血管基病毒基因组9,000碱基段。

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Deletions contained within the genomes of unstable and stable variants of vaccinia virus (strain WR) were analyzed. Restriction endonuclease mapping and hybridization to specific 32P-labeled DNA probes indicated that more than 6 X 10(6) daltons of DNA were deleted from the variants. In each case, the deletion occurred on the left side of the genome and started very close to the junction of the inverted terminal repetition and unique sequence. Both variants also contained a new SstI side on the right side of the genome. Hybridization selection and cell-free translation experiments indicated that these variants lost the ability to synthesize at least eight early mRNA's mapping within the deleted region. Although the deleted DNA was not essential for replication of the WR strain of vaccinia virus under laboratory conditions of infection, it presumably has a defined role under other circumstances. This conclusion was based on the conservation within the Elstree strain of vaccinia, the Utrecht strain of rabbitpox, and the Brighton strain of cowpox virus of sequences homologous to the deleted DNA. Moreover, mRNA's that hybridized to the deleted vaccinia virus DNA segment and encoded similar size polypeptides were made in cells infected with rabbitpox and cowpox viruses.
机译：分析了疫苗病毒（菌株WR）不稳定和稳定变体的基因组内含有的缺失。限制性内切核酸酶对特异性32P标记的DNA探针的映射和杂交表明，从变体中删除了超过6×10（6）个DNA的DNA。在每种情况下，删除发生在基因组的左侧并开始非常接近倒终端重复和独特序列的结。两个变体也包含了基因组右侧的新SSTI侧。杂交选择和无细胞翻译实验表明，这些变体丧失了在删除区域内合成至少八个早期mRNA映射的能力。虽然缺失的DNA对在感染实验室条件下疫苗病毒的WR菌株的复制不是必需的，但可能在其他情况下具有规定的作用。该结论是基于疫苗的紫杉菌菌株的保护，乌没有高温菌株的兔斑菌株，以及与缺失的DNA同源的序列的萌发病毒的荧光菌株。此外，MRNA与缺失的痘苗病毒DNA链段和编码的类似大小多肽杂交的mRNA是在感染的兔斑和豇豆病毒感染的细胞中进行的。

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