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首页> 外文期刊>Journal of Virology >Specific Sindbis virus-coded function for minus-strand RNA synthesis.
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Specific Sindbis virus-coded function for minus-strand RNA synthesis.

机译：用于减去股线RNA合成的特异性Sindbis病毒编码功能。

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The synthesis of minus-strand RNA was studied in cell cultures infected with the heat-resistant strain of Sindbis virus and with temperature-sensitive (ts) belonging to complementation groups A, B, F, and G, all of which exhibited an RNA-negative (RNA-) phenotype when infection was initiated and maintained at 39 degrees C, the nonpermissive temperature. When infected cultures were shifted from 28 degrees C (the permissive temperature) to 39 degrees C at 3 h postinfection, the synthesis of viral minus-strand RNA ceased in cultures infected with ts mutants of complementation groups B and F, but continued in cultures infected with the parental virus and mutans of complementation groups A and G. In cultures infected with ts11 of complementation group B, the synthesis of viral minus-strand RNA ceased, whereas the synthesis of 42S and 26S plus-strand RNAs continued for at least 5 h after the shift to 39 degrees C. However, when ts11-infected cultures were returned to 28 degrees C 1 h after the shift to 39 degrees C, the synthesis of viral minus-strand RNA resumed, and the rate of viral RNA synthesis increased. The recovery of minus-strand synthesis translation of new proteins. We conclude that at least one viral function is required for alphavirus minus-strand synthesis that is not required for plus-strand synthesis. In cultures infected with ts6 of complementation group F, the syntheses of both viral plus-strand and minus-strand RNAs were drastically reduced after the shift to 39 degrees C. Since ts6 failed to synthesize both plus-strand and minus-strand RNAs after the shift to 39 degrees C, at least one common viral component appears to be required for the synthesis of both minus-strand and plus-strand RNAs.
机译：研究了在感染的Sindbis病毒耐热菌株和属于互补组A，B，F和G的温度敏感（TS）的细胞培养物中的合成，所有这些都表现出RNA-在感染时引发并保持在39℃，非易感温度时，阴性（RNA-）表型。当感染的培养物在3小时后从28℃（允许温度）转移到39摄氏度时，在感染的TS突变体的培养物中停止的病毒减去股线RNA的合成，但在感染的培养物中继续与父母病毒和互补组的异常A和G.在感染互补组B的TS11中，持续的病毒减去股RNA的合成，而42℃和26s加股的合成持续至少5小时然而，在换档到39℃后将TS11感染的培养物恢复到28℃1小时后，恢复了病毒减去股线RNA的合成，并且病毒RNA合成的合成增加。新蛋白的负链合成翻译的回收。我们得出结论，alphavirus减去链合成需要至少一种病毒功能，其不需要加号合成。在感染互补组F的TS6的培养物中，在移至39℃后，病毒加股和减去股线的合成急剧减少。由于TS6未能在后面的情况下合成正链和减去股线rNA向39℃变为39℃，似乎至少一个常见的病毒组分似乎是合成减去股和股线RNA的。

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