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首页> 外文期刊>Journal of Virology >Evidence for an internal component of the bacteriophage T4D tail core: a possible length-determining template.
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Evidence for an internal component of the bacteriophage T4D tail core: a possible length-determining template.

机译：噬菌体T4D尾部内部组分的证据：可能的长度测定模板。

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The length of the T4 tail is precisely regulated in vivo at the time of polymerization of the tail core protein onto the baseplate. Since no mutations which alter tail length have been identified, a study of in vivo-assembled tail cores was begun to determine whether the structural properties of assembled cores would reveal the mechanism of length regulation. An assembly intermediate consisting of a core attached to a baseplate (core-baseplate) was purified from cells infected with a T4 mutant in gene 15. When core-base plates were treated with guanidine hydrochloride, cores were released from baseplates. The released cores had the same mean length as cores attached to baseplates. Electron micrographs of these cores showed partial penetration of negative stain into one end, and, at the opposite end, a modified tip which often appeared as a short fiber projecting from the core. When cores were purified and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, two minor proteins and the major core protein were detected. One minor protein, the product of gene 48 (gp48), was present in at least 72% of the amount found in core-baseplates, relative to the amount of the major core protein. These findings suggest that cores contain a fibrous structure, possibly composed of gp48, which may form a "ruler" that specifies the length of the T4 tail.
机译：T4尾的长度在尾核蛋白的聚合时精确地调节在尾部核心蛋白到底板上。由于没有识别出尾长的突变，因此开始研究体内尾部的研究，以确定组装核心的结构性能是否会揭示长度调节的机制。由在基因15中感染的细胞中纯化由附着在底板（芯底板）的芯组成的组装中间体。当用盐酸胍处理核基板时，核心从底板中释放。释放的核心具有与连接到底板的核心相同的平均长度。这些芯的电子显微照片显示出负污渍的部分穿透到一端，并且在相对的端部，经常出现作为从芯突出的短纤维的改进的尖端。当通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳纯化并分析核心时，检测到两种次要蛋白质和主要核心蛋白。一个次要的蛋白质，基因48（GP48）的产物存在于相对于主要核心蛋白的量的核底板中发现的至少72％。这些发现表明，核心含有纤维结构，可能由GP48组成，其可以形成“标尺”，其指定T4尾部的长度。

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