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首页> 外文期刊>Journal of Virology >Evidence that infectious pancreatic necrosis virus has a genome-linked protein.
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Evidence that infectious pancreatic necrosis virus has a genome-linked protein.

机译：有证据表明传染性胰腺坏死病毒具有基因组连接蛋白质。

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The double-stranded RNA segments of infectious pancreatic necrosis virus were extracted from virions by a method which avoids proteinase. In contrast to proteinase-treated RNA, such segments (i) exhibited a lower electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels and agarose gels, (ii) had a slightly lower buoyant density, and (iii) demonstrated a marked tendency toward aggregation as observed by electron microscopy. A small amount of protein tightly bound to the RNA could account for the above properties, and a 110,000-dalton protein was liberated from purified virion RNA by sequential digestion with RNase III and RNase A. The amount of radioactivity associated with RNA from virions labeled in vivo with [35S]methionine suggested that an average of 1.4 molecules was bound per RNA segment. Interactions between RNA segments seen in electron micrographs appeared to occur only among the ends of the segments, suggesting these were the exclusive sites of protein attachment.
机译：通过避免蛋白酶的方法从病毒粒中提取传染性胰腺坏死病毒的双链RNA片段。与蛋白酶处理的RNA相反，这种段（I）在十二烷基硫酸钠 - 聚丙烯酰胺凝胶中表现出较低的电泳迁移率，并且琼脂糖凝胶具有稍微较低的浮力密度，并且（iii）证明了朝向聚集的显着趋势通过电子显微镜观察。少量与RNA紧密结合的蛋白质可以解释上述性质，通过用RNase III和RNase A顺序消化从纯化的病毒虫RNA中释放出110,000-Dalton蛋白。与标记的病毒粒子相关的放射性量的放射性量具有[35s]蛋氨酸的体内表明每次RNA段平均约束1.4分子。在电子显微照片中看到的RNA段之间的相互作用仅在段的末端发生，表明这些是蛋白质附着的独家位点。

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