Yeast RNA polymerase I binds preferentially to A+T-rich linkers in rDNA

Odd Stokke Gabrielsen; Tordis B. ?yen

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Yeast RNA polymerase I binds preferentially to A+T-rich linkers in rDNA

机译：酵母RNA聚合酶I优先结合到RDNA中的+ T-富含的接头

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Restriction fragments of yeast rDNA retained by purified RNA polymerases on nitrocellulose filters were analysed by gel eleotrophoresis. The EcoRI fragment B was preferentially retained by RNA polymerise I, but not by RNA polymerase III. The in vivo initiation sites for both polymerases are located within this fragment. Further analysis indicated that the preferred binding site for RNA polymerase I is highly AT-rich regions rather than a true promoter. The reported selective in vitro transcription of rDNA by purified yeast RNA polymerase I could then be explained by this preferential binding.

机译：通过凝胶Eleootrophoreris分析通过纯化的RNA聚合酶保留在硝酸纤维素过滤器上保留的酵母RDNA的限制片段。 ECORI片段B优先由RNA聚合I保留，但不是由RNA聚合酶III保留。两种聚合酶的体内起始位点位于该片段内。进一步的分析表明，RNA聚合酶I的优选结合位点I具有高度富有的区域，而不是真正的启动子。通过纯化的酵母RNA聚合酶I，通过纯化的酵母RNA聚合酶I可以解释所报道的rDNA的体外转录。

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《Nucleic acids research》 |1982年第19期|共12页
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Odd Stokke Gabrielsen; Tordis B. ?yen;
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收录信息美国《科学引文索引》(SCI);
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1. Yeast RNA polymerase I binds preferentially to A+T-rich linkers in rDNA [J] . Odd Stokke Gabrielsen, Tordis B. ?yen Nucleic acids research . 1982,第19期

机译：酵母RNA聚合酶I优先结合到RDNA中的+ T-富含的接头
2. Yeast RNA polymerase I binds preferentially to A+T-rich linkers in rDNA [J] . Odd Stokke Gabrielsen, Tordis B. ?yen Nucleic acids research . 1982,第19期

机译：酵母RNA聚合酶I优先结合到RDNA中的+ T-富含的接头
3. The fission yeast RPA21 subunit of RNA polymerase I: an evolutionarily conserved subunit interacting with ribosomal DNA (rDNA) transcription factor Rrn3p for recruitment to rDNA promoter [J] . Imazawa Y, Hisatake K, Nakagawa K, Genes & Genetic Systems . 2002,第3期

机译：RNA聚合酶I的裂变酵母RPA21亚基：与核糖体DNA（rDNA）转录因子Rrn3p相互作用的进化保守亚基，可募集到rDNA启动子
4. Proteomics mapping of Yeast RNA Polymerase II Post-translational modifications and investigating their biological significance [C] . Sreenivasa Rao Ramisetty, Amber Mosley, Michael P. Washburn American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：酵母RNA聚合酶II的蛋白质组学测绘后翻译后修饰和研究其生物学意义
5. Regulation of the HCV RNA-dependent RNA polymerase NS5B by the HCV capsid protein and characterization of the NS5B juxtamembrane linker [D] . Wen, Yahong. 2014

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6. Yeast RNA polymerase I binds preferentially to A+T-rich linkers in rDNA. [O] . O S Gabrielsen, T B Oyen 1982

机译：酵母RNA聚合酶I优先结合rDNA中富含A + T的接头。
7. Yeast RNA polymerase I binds preferentially to A+T-rich linkers in rDNA. [O] . Gabrielsen, O S, Oyen, T B 1982

机译：酵母RNA聚合酶I优先结合rDNA中富含A + T的接头。

Yeast RNA polymerase I binds preferentially to A+T-rich linkers in rDNA

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