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首页> 外文期刊>Journal of Virology >Isolation of vesicular stomatitis virus defective interfering genomes with different amounts of 5'-terminal complementarity.
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Isolation of vesicular stomatitis virus defective interfering genomes with different amounts of 5'-terminal complementarity.

机译:隔离囊泡口炎病毒有缺陷的干扰基因组,不同量的5'末端互补性。

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I isolated at least 30 different vesicular stomatitis virus defective interfering (DI) genomes, distinguished by chain length, by five independent undiluted passages of a repeatedly cloned virus plaque. Labeling of the 3' hydroxyl ends of these DI genomes and RNase digestion studies demonstrated that the ends of these DI genomes were terminally complementary to different extents (approximately 46 to 200 nucleotides). Mapping studies showed that the complementary ends of all of the DI genomes were derived from the 5' ends of the nondefective minus-strand genome. Regardless of the extent of terminal complementarity, all of the DI genomes synthesized the same 46-nucleotide minus-strand leader RNA.
机译:我孤立至少30种不同的囊泡口炎病毒有缺陷的干扰(DI)基因组,通过链长的分类,通过反复克隆的病毒斑块的五个独立的未稀释通道。这些DI基因组和RNase消化研究的3'羟基末端的标记表明,这些DI基因组的末端与不同的范围(约46〜200个核苷酸)末端互补。映射研究表明,所有DI基因组的互补末端均来自NondFective减去股基因组的5'末端。无论终端互补程度如何,所有的DI基因组都合成了相同的46核苷酸减去链引导RNA。

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  • 来源
    《Journal of Virology》 |1982年第2期|共9页
  • 作者

    D Kolakofsky;

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  • 收录信息 美国《科学引文索引》(SCI);
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