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首页> 外文期刊>Nucleic acids research >Analysis of chromatin structure and DNA sequence organization: use of the 1,10-phenanthroline-cuprous complex
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Analysis of chromatin structure and DNA sequence organization: use of the 1,10-phenanthroline-cuprous complex

机译：染色质结构和DNA序列组织分析：使用1,10菲咯啉 - 铜络合物

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Limited treatment of Drosophila nuclei with the 1,10-phenanthroline-cuprous complex leads to rapid production of nucleosomal ladders indistinguishable from those obtained by micrococcal nuclease digestion. An investigation of the preferential sites of cleavage of protein-free DNA at locus 67B1 surprisingly indicated that both reagents recognized very similar features. Thus, a virtually identical pattern of preferential cleavages was generated over a 12 kb fragment encoding four transcripts at this locus. The distribution of cleavage sites was highly non-random, with major sites falling in the spacers between the genes. Both reagents cleaved certain chromatin-specific sites near the 5′ ends of the genes. However, an analysis of preferential cleavages at the sequence level did not reveal the same close correspondence. We suggest that both reagents can recognize some localized secondary structural features of the DNA and that the particular distribution of sequences present at this locus results in a distinctive pattern of cleavage sites that delineates gene and spacer segments.
机译：与1,10菲碱 - 亚铜复合物的果蝇核的有限处理导致快速生产核致梯子，与通过微球菌核酸酶消化获得的核心梯子难以区分。令人遗憾的是对位于洛杉矶67B1的无蛋白质DNA切割的优先网站的研究令人惊讶地表明，两种试剂都识别出非常相似的特征。因此，在该基因座上编码四个转录物的12kb片段产生几乎相同的优先裂解模式。分裂位点的分布非常非随机，主要部位落入基因之间的间隔物。两种试剂在基因的5'末端附近切割某些染色质特异性位点。然而，在序列级别的优先性切割的分析没有揭示相同的密钥。我们认为两种试剂可以识别DNA的一些局部二次结构特征，并且在该基因座处存在的特定分布导致划清基因和间隔区段的裂解位点的独特模式。

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