Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3.small ribosomal subunit, as shown by cross-linking experiments

Odd Nyg?rd; Peter Westermann

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Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3.small ribosomal subunit, as shown by cross-linking experiments

机译：一种真核激素eIF-3的一个亚基与18S核糖体RNA在二元络合物中的一个亚基的比相互作用，EIF-3.Small核糖体亚基，如交联实验所示

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Initiation factor eIF-3 from rat liver forms a binary complex with the small ribosomal subunit. Within this complex, 18S ribosomal RNA can be cross-linked to the 66 000 dalton subunit of eIF-3 by treating the complex with a short bifunctional reagent, diepoxybutane, with a distance of 4A between the reactive groups. In binary complexes containing eIF-3 premodified with the heterobifunctional reagent, methyl-p-azido-benzoylaminoacetimidate (10?), the 66 000 dalton subunit of eIF-3 became covalently bound to 18S rRNA after irradiation of the complex with ultraviolet light. The involvement of only one of the eight eIF-3 subunits in the formation of the covalent RNA-protein complexes indicates a highly specific interaction between 18S rRNA and eIF-3 at the attachment site of the factor on the 40S subunit.

机译：来自大鼠肝脏的启动因子EIF-3与小核糖体亚基形成二元络合物。在该络合物中，通过用短的双官能试剂，Diepoxybutane处理复合物，18S核糖体RNA可以与EIF-3的66 000dalton亚基交联，偏氧丁烷在反应基团之间具有4A的距离。在含有异双官能试剂的EIF-3的二元络合物中，在用紫外线照射复合物照射后，EIF-3的66 000dalton亚基，EIF-3的66 000dalton亚基在紫外线照射后，EIF-3的66 000大氏亚基。在共价RNA蛋白复合物的形成中仅在形成共价RNA蛋白质复合物的形成中的涉及表明，在40s亚基的因子的附着部位，18s rRNA和EIF-3之间的高度比相互作用。

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《Nucleic acids research》 |1982年第4期|共8页
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Odd Nyg?rd; Peter Westermann;
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1. Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3.small ribosomal subunit, as shown by cross-linking experiments [J] . Odd Nyg?rd, Peter Westermann Nucleic acids research . 1982,第4期

机译：一种真核激素eIF-3的一个亚基与18S核糖体RNA在二元络合物中的一个亚基的比相互作用，EIF-3.Small核糖体亚基，如交联实验所示
2. Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3.small ribosomal subunit, as shown by cross-linking experiments [J] . Odd Nyg?rd, Peter Westermann Nucleic acids research . 1982,第4期

机译：一种真核激素eIF-3的一个亚基与18S核糖体RNA在二元络合物中的一个亚基的比相互作用，EIF-3.Small核糖体亚基，如交联实验所示
3. The α and γ subunits of initiation factor elF-2 can be cross-linked to 18S ribosomal RNA within the quaternary initiation complex, eIF-2-Met-tRNAf GDPCP small ribosomal subunit [J] . Heinz Bielka, Odd Nygārd, Peter Westermann Nucleic acids research . 1980,第14期

机译：起始因子eIF-2的α和γ亚基可与四元起始复合物eIF-2-Met-tRNAf GDPCP小核糖体亚基内的18S核糖体RNA交联。
4. Small Subunit Ribosomal RNA Modeling Using Stochastic Context-Free Grammars [C] . Michael P.S. Brown International Conference on Intelligent Systems for Molecular Biology; 20000816-23; La Jolla,CA(US) . 2000

机译：小亚基核糖体RNA建模使用随机上下文无关文法
5. The end of the beginning: Eukaryotic ribosomal subunit joining and the combined roles of initiation factors 5B and 1A. [D] . Acker, Michael Gerard. 2009

机译：开始的结尾：真核生物核糖体亚基的结合以及起始因子5B和1A的共同作用。
6. Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex eIF-3 small ribosomal subunit as shown by cross-linking experiments. [O] . O Nygård, P Westermann 1982

机译：真核起始因子eIF-3的一个亚基与二元复合体eIF-3小核糖体亚基内的18S核糖体RNA的特异性相互作用如交联实验所示。
7. Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3 small ribosomal subunit, as shown by cross-linking experiments. [O] . Nygård, O, Westermann, P 1982

机译：真核起始因子eIF-3的一个亚基与二元复合体eIF-3小核糖体亚基内的18S核糖体RNA的特异性相互作用，如交联实验所示。

Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3.small ribosomal subunit, as shown by cross-linking experiments

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