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首页> 外文期刊>Journal of Virology >Further characterization of mRNA's of mouse hepatitis virus: presence of common 5'-end nucleotides.
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Further characterization of mRNA's of mouse hepatitis virus: presence of common 5'-end nucleotides.

机译:小鼠肝炎病毒mRNA的进一步表征:共同的5'-末端核苷酸的存在。

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The mouse hepatitis virus strain A59 codes for seven RNA species in the infected cells. These virus-specific RNAs were found to be polysome associated and therefore likely to represent mRNA's. All of them have common 3'-end sequences (Lai et al., J. Virol. 39:823-834, 1981). Their structure was further studied with respect to their 5'-end sequences. It was found that all of these mRNA's contained cap structures at their 5' ends. Furthermore, the cap-containing oligonucleotides which represent the sequences immediately adjacent to the 5' ends were found to be the same for most, if not all, of the seven virus-specific mRNA's. These sequences are also identical to the 5'-end sequences of the virion RNA genome. The 5'-end sequences were tentatively determined to be 5'-cap-N-UAAG. The presence of the common nucleotides in all of the virus-specific RNAs in mouse hepatitis virus strain A59 suggests several possible mechanisms of synthesis for these RNAs. The significance of these findings is discussed.
机译:小鼠肝炎病毒菌株A59代码在感染细胞中的七种RNA种类。发现这些病毒特异性的RNA是多肌气相关的,因此可能代表mRNA。所有这些都有常见的3'-结束序列(Lai等,J.Virol。39:823-834,1981)。相对于其5'-末端序列进一步研究了它们的结构。发现所有这些MRNA都在其5'末端包含盖帽结构。此外,发现含帽的寡核苷酸,其表示邻近5'末端的序列的序列与七种病毒特异性mRNA中的大多数情况相同。这些序列也与病毒虫RNA基因组的5'-末端序列相同。暂时测定5'-末端序列是5'-cap-n-uaag。小鼠肝炎病毒菌株A59中所有病毒特异性RNA中的常见核苷酸的存在表明这些RNA的几种可能的合成机制。讨论了这些发现的重要性。

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