Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti plasmld-encoded tmr gene

F. Heidekamp; H. van Ormondt; J. Hille; W.G. Dirkse

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Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti plasmld-encoded tmr gene

机译：土壤杆菌章鱼章节章鱼Ti Prasmld-Cododed TMR基因的核苷酸序列

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The nucleotide sequence of the tmr gene, encoded by the octopine Ti plasmid from Agrobacterium tumefaciens (pTiAch5), was determined. The T-DNA, which encompasses this gene, is Involved in tumor formation and maintenance, and probably mediates the cytokinin-independent growth of transformed plant cells. The nucleotide sequence of the tmr gene displays a continuous open reading frame specifying a polypeptide chain of 240 amino acids. The 5′- terminus of the polyadenylated tmr mRNA isolated from octopine tobacco tumor cell lines was determined by nuclease SI mapping. The nucleotide sequence 5′-TATAAAA-3′, which sequence is Identical to the canonical “TATA” box, was found 29 nucleotides upstream from the major initiation site for RNA synthesis. Two potential poly-adenylatlon signals 5′-AATAAA-3′ were found at 207 and 275 nucleotides downstream from the TAG stopcodon of the tmr gene. A comparison was made of nucleotide stretches, involved in transcription control of T-DNA genes.

机译：测定由官僚曲调（PTAIACH5）的章鱼Ti质粒编码的TMR基因的核苷酸序列。包含该基因的T-DNA参与肿瘤形成和维护，并且可能介导转化的植物细胞的细胞素无关的生长。 TMR基因的核苷酸序列显示出指定240个氨基酸的多肽链的连续开放读数框。通过核酸酶Si测绘测定从章鱼烟草肿瘤细胞系中分离的多腺苷酸化TMR mRNA的5'末端。序列5'-tataAA-3'，其序列与规范“Tata”盒相同，发现来自主要引发位点的29个核苷酸，用于RNA合成。在TMR基因的标签中，在207和275个核苷酸中发现了两个潜在的聚亚乙酰丙酮信号5'-AataAA-3'。比较由核苷酸延伸制成，参与T-DNA基因的转录控制。

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《Nucleic acids research》 |1983年第18期|共14页
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F. Heidekamp; H. van Ormondt; J. Hille; W.G. Dirkse;
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收录信息美国《科学引文索引》(SCI);
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1. Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti plasmld-encoded tmr gene [J] . F. Heidekamp, H. van Ormondt, J. Hille, Nucleic acids research . 1983,第18期

机译：土壤杆菌章鱼章节章鱼Ti Prasmld-Cododed TMR基因的核苷酸序列
2. Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti plasmld-encoded tmr gene [J] . F. Heidekamp, H. van Ormondt, J. Hille, Nucleic acids research . 1983,第18期

机译：土壤杆菌章鱼章节章鱼Ti Prasmld-Cododed TMR基因的核苷酸序列
3. Negative control of octopine degradation and transfer genes of octopine Ti plasmids in Agrobacterium tumefaciens. [J] . P M Klapwijk, R A Schilperoort Journal of bacteriology . 1979,第2期

机译：根癌农杆菌中对章鱼碱降解和章鱼碱Ti质粒质粒转移基因的负控制。
4. EFFECTS OF BACTERIAL BLIGHT RESISTANCE GENES AND AGROBACTERIUM TUMEFACIENS VIRULENCE ON DEVELOPMENT OF BRONZE WILT SYMPTOMS AND COTTON YIELD LOSSES [C] . Alois A. Bell Beltwide Cotton Conference . 2003

机译：细菌枯萎基因和土壤杆菌毒力毒力对青铜枯萎病症状和棉花产量损失的影响
5. Identification of new pathogenicity genes in Magnaporthe oryzae through the construction of an Agrobacterium tumefaciens-mediated insertion mutant library. [D] . Betts, Melania Figueroa. 2007

机译：通过根癌农杆菌介导的插入突变体文库的构建，鉴定稻米中新的致病基因。
6. Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti plasmid-encoded tmr gene. [O] . F Heidekamp, W G Dirkse, J Hille, 1983

机译：根癌农杆菌章鱼碱Ti质粒编码的tmr基因的核苷酸序列。
7. Nucleotide sequence of the virulence gene virG of the Agrobacterium tumefaciens octopine Ti plasmid: significant homology between virG and the regulatory genes ompR, phoB and dye of E. coli. [O] . Melchers, L S, Thompson, D V, Idler, K B, 1986

机译：根癌农杆菌章鱼碱Ti质粒的毒力基因virG的核苷酸序列：virG与大肠杆菌的调控基因ompR，phoB和染料之间具有显着同源性。

Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti plasmld-encoded tmr gene

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