...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Stoichiometry of large T antigen and pp53 in complexes isolated from simian virus 40-transformed rat cells.
【24h】

Stoichiometry of large T antigen and pp53 in complexes isolated from simian virus 40-transformed rat cells.

机译：硅藻病毒40转化大鼠细胞中分离的复合物中大T抗原和PP53的化学计量。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Simian virus 40-transformed cells synthesize high-molecular-weight protein complexes (22 to 30S) that consist of the virus-coded large T antigen (81,500 daltons) and the cellular antigen pp53. These complexes were partially purified from lysates of transformed rat cells by sucrose velocity sedimentation. The stoichiometry of the two proteins in the complex was studied by direct enzyme-linked immunosorbent assays, using alkaline phosphatase-conjugated anti-T and anti-pp53 monoclonal antibodies. The results from these experiments indicate that the T antigen-to-pp53 ratio in the complex is 0.87 +/- 0.27. No statistically significant differences were found in this ratio for faster- and slower-sedimenting complexes. These results from enzyme-linked immunosorbent assays and previous molecular weight estimates of the complex suggest that this complex is composed, on the average, of four molecules of T antigen and four or five molecules of pp53.
机译：Simian病毒40-转化的细胞合成高分子量蛋白质复合物（22至30秒），其由病毒编码的大T抗原（81,500道尔顿）和细胞抗原PP53组成。通过蔗糖速度沉降部分从转化的大鼠细胞的裂解物中部分纯化这些复合物。通过直接酶联免疫吸附试验研究复合物中两种蛋白质的化学计量，使用碱性磷酸酶 - 缀合的抗-T和抗PP53单克隆抗体。这些实验的结果表明复合物中的T抗原-PP53比为0.87 +/- 0.27。在这种比率中没有发现统计学上的显着差异，以更快，沉淀沉降沉降。这些来自酶联免疫吸附测定的结果和复合物的先前分子量估计表明该络合物在平均四分之一的T抗原和四个或五个PP53分子中组成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号