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首页> 外文期刊>Journal of bacteriology >Regulation of folylpoly-gamma-glutamate synthesis in bacteria: in vivo and in vitro synthesis of pteroylpoly-gamma-glutamates by Lactobacillus casei and Streptococcus faecalis.
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Regulation of folylpoly-gamma-glutamate synthesis in bacteria: in vivo and in vitro synthesis of pteroylpoly-gamma-glutamates by Lactobacillus casei and Streptococcus faecalis.

机译：细菌中福利-Gomat-gamma-谷氨酸合成的调节：在体内和体外合成乳酸杆菌和链球菌雌核苷酸的戊酰胺 - 谷氨酸。

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Lactobacillus casei and Streptococcus faecalis accumulated labeled folic acid and metabolized this compound to poly-gamma-glutamates of chain lengths of up to 11 and 5, respectively. Octa- and nonaglutamates predominated in L. casei, and tetraglutamates predominated in S. faecalis. The most effective monoglutamate substrates for the L. casei and S. faecalis folylpoly-gamma-glutamate (folylpolyglutamate) synthetases were methylene- and formyltetrahydrofolate, respectively. Methylenetetrahydropteroylpoly-gamma-glutamates were the preferred poly-gamma-glutamate substrates for both enzymes and, in each case, the highest activity was observed with the diglutamate substrate. The final distribution of folylpolyglutamates in these bacteria appeared to reflect the ability of folates with various glutamate chain lengths to act as substrates for the bacterial folylpolyglutamate synthetases. The proportions of individual folylpolyglutamates were markedly affected by culturing the bacteria in medium containing adenine, whereas thymine was without effect. Adenine did not affect the level of folylpolyglutamate synthetase in either organism but caused a large increase in the proportion of intracellular folates containing one-carbon units at the oxidation level of formate, folates which are substrates for enzymes involved in purine biosynthesis. The folates with shorter glutamate chain lengths in bacteria cultured in the presence of adenine resulted from primary regulation of the de novo purine biosynthetic pathway, regulation which caused an accumulation of formyltetrahydropteroyl-poly-gamma-glutamates (folate derivatives that are ineffective substrates for folylpolyglutamate synthetases), and did not result from regulation of folylpolyglutamate synthetase per se.
机译：乳杆菌酪虫和链球菌粪便分别累积标记的叶酸，并将该化合物代谢至聚链长度高达11和5的聚γ-谷氨酸。在L.酪虫中占主导地位的八烷基和非醛酸盐和粪便中的四氟醚。 L.酪虫和粪醛福利-Gamma-谷氨酸（福利戊酰胺）合成酶的最有效的单戊磺酸酯底物分别是亚甲基和甲酰基四氢甲酸酯。甲基四氢吡喃酮酰氯聚乙烯 - γ-谷氨酸是用于两种酶的优选的多γ-谷氨酸底物，并且在每种情况下，用碳水化合物基材观察到最高活性。这些细菌中福利戊酰胺的最终分布似乎反映了叶酸与各种谷氨酸链长度的能力，以充当细菌福利谷氨酸合成酶的基材。通过培养含腺嘌呤的培养基中的细菌，胸腺嘧啶的含量显着影响单个福利谷氨酸的比例显着影响。腺嘌呤不影响任何一种生物体中福利聚氨酸盐酯的水平，而是引起含有单碳单位的细胞内叶片的比例的大幅增加，甲酸甲酸水平，叶片是嘌呤生物合成的酶酶的底物。在腺嘌呤存在下培养的细菌中具有较短的谷氨酸链长度的叶片导致DE Novo嘌呤生物合成途径的初次调节，导致甲酰基四水酰酰基聚伽马酰胺酰胺酰胺的累积（叶酸衍生物为福利氟酯酰胺合成酶的叶酸衍生物），并未导致对福利谷氨酸合成酶的调节本身。

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