Proton NMR studies on the covalently linked RNA-DNA hybrid r(GCG)d(TATACGO). Assignment of proton resonances by application of the nuclear Overhauser effect

C. Altona; C.A.A. van Boeckel; C.A.G. Haasnoot; G. Wille; G.A. van der Marel; J.-R. Mellema; J.H. van Boom

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Proton NMR studies on the covalently linked RNA-DNA hybrid r(GCG)d(TATACGO). Assignment of proton resonances by application of the nuclear Overhauser effect

机译：质子NMR研究共价连接的RNA-DNA杂交r（GCG）D（TATACGO）。通过应用核传承效应分配质子共振

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Proton NMR spectra of a covalently linked self-complementary RNA?DNA hybrid, r(GCG)-d(TATACGC), are recorded in H₂O and D₂O. Imino proton resonances as well as the non-exchangeable base and H-1' resonances are unambiguously assigned by means of nuclear Overhauser effect measurements. Additional information was obtained by ₂₁P NMR and circular dichroism spectra. The RNA parts in the duplex attain full conformational purity and adopt the usual A-RNA conformation. The DNA residues opposite the RNA tract do not adopt an A-type structure completely. Their respective sugar rings still appear to possess a certain conformational freedom. The same holds true for the central d(-TATA-) sequence which forms a DNA?DNA duplex. There appears to be a structural break in this part: the first two residues, T(4) and A(5), are clearly influenced by the adjacent RNA structure, whereas residues T(6) and A(7) behave quite similar to what usually is found in DNA duplexes in aqueous solution.

机译：共价连接的自互补RNA培养物的质子NMR光谱DNA杂合物R（GCG）-D（TATACGC）被记录在H _{2 O和D _{2 O中。亚氨基质子共振以及不可交换的基部和H-1'共振通过核传承效应测量明确分配。通过_{21 p NMR和圆形二色性谱获得附加信息。双面的RNA部件达到全构象纯度并采用通常的A-RNA构象。与RNA道相对的DNA残基不会完全采用A型结构。它们各自的糖戒指仍然具有一定的构象自由。对于形成DNA的中央D（-TATA-）序列，相同的保持真实ΔDNA双链体。在该部分中似乎是一种结构突破：前两个残基，T（4）和A（5）被相邻的RNA结构明显影响，而残留物T（6）和a（7）表现得非常相似通常在水溶液中的DNA双链体中发现了什么。}}}

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《Nucleic acids research》 |1983年第16期|共22页
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C. Altona; C.A.A. van Boeckel; C.A.G. Haasnoot; G. Wille; G.A. van der Marel; J.-R. Mellema; J.H. van Boom;
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1. Proton NMR studies on the covalently linked RNA-DNA hybrid r(GCG)d(TATACGO). Assignment of proton resonances by application of the nuclear Overhauser effect [J] . C. Altona, C.A.A. van Boeckel, C.A.G. Haasnoot, Nucleic acids research . 1983,第16期

机译：质子NMR研究共价连接的RNA-DNA杂交r（GCG）D（TATACGO）。通过应用核传承效应分配质子共振
2. Proton NMR studies on the covalently linked RNA-DNA hybrid r(GCG)d(TATACGO). Assignment of proton resonances by application of the nuclear Overhauser effect [J] . C. Altona, C.A.A. van Boeckel, C.A.G. Haasnoot, Nucleic acids research . 1983,第16期

机译：质子NMR研究共价连接的RNA-DNA杂交r（GCG）D（TATACGO）。通过应用核传承效应分配质子共振
3. Nuclear magnetic resonance studies on yeast tRNAPhe.II. Assignment of the iminoproton resonances of the anticodon and T stem by means of nuclear Overhauser effect experiments at 500 MHz [J] . A. Heerschap, C.A.G. Haasnoot, C.W. Hilbers Nucleic acids research . 1983,第13期

机译：酵母tRNAPhe的核磁共振研究II。通过500 MHz的核Overhauser效应实验分配反密码子和T茎的亚氨基质子共振
4. Proton-Deuteron Amide Exchange Studies by MS and NMR: a New Method for Protein NMR Resonance Assignment [C] . Lianmei Feng, James H. Prestegard, Ron Orlando ASMS Conference on Mass Spectrometry and Allied Topics . 2006

机译：Proton-Deuteron酰胺Exchange研究MS和NMR：蛋白质NMR共振分配的一种新方法
5. Solution-state proton nuclear magnetic resonance (NMR) spectroscopic studies of the active site of myoglobins in various ligated states: Models for macromolecule-substrate binding and advancement of paramagnetic NMR techniques. [D] . Yee, Sidney. 1993

机译：各种结扎状态下肌球蛋白活性位点的溶液状态质子核磁共振（NMR）光谱研究：高分子-底物结合模型和顺磁NMR技术的发展。
6. Proton NMR studies on the covalently linked RNA-DNA hybrid r(GCG)d(TATACGC). Assignment of proton resonances by application of the nuclear Overhauser effect. [O] . J R Mellema, C A Haasnoot, G A van der Marel, 1983

机译：对共价连接的RNA-DNA杂合体r（GCG）d（TATACGC）进行质子NMR研究。通过应用核Overhauser效应分配质子共振。
7. Nuclear magnetic resonance studies on yeast tRNAPhe. III. Assignments of the iminoproton resonances of the tertiary structure by means of nuclear Overhauser effect experiments at 500 MHz. [O] . Heerschap, A, Haasnoot, C A, Hilbers, C W 1983

机译：酵母tRNAPhe的核磁共振研究。三，通过在500 MHz的核Overhauser效应实验确定三级结构的亚氨基质子共振。

Proton NMR studies on the covalently linked RNA-DNA hybrid r(GCG)d(TATACGO). Assignment of proton resonances by application of the nuclear Overhauser effect

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