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首页> 外文期刊>Nucleic acids research >A mammalian tRNA His-Containing antigen is recognized by the polymyosits-specific antibody antijo-1
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A mammalian tRNA His-Containing antigen is recognized by the polymyosits-specific antibody antijo-1

机译：哺乳动物TRNA抗原的抗原被特异性抗体的抗毒性抗-1识别

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SUMMARY The mammalian cell antigen reactive with the autoantibody anti-jo-1 has been shown to contain tRNARis. The RNA sequence of this human and mouse cell tRNA was determined in a search for unusual features that might be realted to antigenicity. The 5' terminal nuleotide is unique among other sequenced tRNAs in that it is a methylated guanine. The presence of the hypermodified base quenine, which occurs in the wobble postition of the anticodon of tRNAHis from several species, was not detected in the tRNAHis immunoprecipitated by anti-jo-1 from either human HeLa or mouse Friend erytholeukeia cell extracts. The binding of protien(s) appears to confer antigenicity on tRNAHis since either protienase K treatment or phenol extraction resulted in the loss of immunoprecipatability. However, we have not suceeded in identifying an antigenic protien, and we find that the antigenic complex is not resolved from purified tRNAHis by Sepharcryl S-200 column chromatography. Immunofluorescence studies indicate that the antigenic from of tRNAHis is located preferentially in the mammalian cell cytoplasm. The results presented here are discussed in light of an earlier report (1) on the nature of the jo-1 antigen.
机译：发明内容已显示与自身抗体抗-J-1反应的哺乳动物细胞抗原含有TRNA RIS 。在寻求可能与抗原性的寻常特征中测定该人和小鼠细胞TRNA的RNA序列。 5'末端Nuleotide在其他测序的TrNA中是独一无二的，因为它是甲基化的鸟嘌呤。在TRNA HIM 免疫沉淀的TRN​​A 他的抗侵略声的抗ordon的抗ordon的抗oraton的抗ordon的抗oraton的抗ordon的抗ordon的抗oraton的抗oraton的抗oraton的发布中存在的存在。 -1来自人类的Hela或小鼠朋友红绿细胞提取物。蛋白酶的结合似乎在TRNA上赋予抗原性他的，因为蛋蛋白酶K处理或苯酚提取导致免疫沉积性的损失。然而，我们没有成功鉴定抗原蛋白，并且发现抗原复合物未通过Sepharcryl S-200柱色谱法从纯化的Trna 他的中解剖。免疫荧光研究表明，TRNA HIS 的抗原性优先位于哺乳动物细胞细胞质中。这里介绍的结果是根据早期的报告（1）对Jo-1抗原的性质进行讨论。

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