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首页> 外文期刊>Journal of Virology >Isolation of episomal bovine papillomavirus chromatin and identification of a DNase I-hypersensitive region.
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Isolation of episomal bovine papillomavirus chromatin and identification of a DNase I-hypersensitive region.

机译：异常牛乳头瘤病毒染色质的分离及鉴定DNase I-过敏区。

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The investigation of papillomavirus chromatin has been hampered by the unavailability of a tissue culture system for vegetative growth of these viruses. We have used, therefore, bovine papillomavirus type 1-transformed hamster embryo fibroblasts containing 200 to 250 episomal genome equivalents per cell as a source of viral chromatin. The selectively isolated chromatin was shown to be slightly larger (80S) than the mature simian virus 40 chromatin, which was cosedimented in a sucrose density gradient. Both Fo I and Fo II were present in the bovine papillomavirus type 1 chromatin. A fast-sedimenting fraction, whose structure is still unknown, also contained oligomeric bovine papillomavirus type 1 DNA. By in situ DNase digestion of isolated nuclei and subsequent cleavage of the bovine papillomavirus type 1 DNA with various restriction endonucleases, a major DNase-hypersensitive region was detected in the chromatin. This region, comprising approximately 320 base pairs, is located between the relative physical map positions 0.88 and 0.92.
机译：通过组织培养系统对这些病毒的营养生长的不可用的不可用的乳头瘤病毒染色质的调查受到阻碍。因此，我们已经使用，因此，每种细胞含有200至250个重组基因组当量的1转变化的仓鼠胚胎成纤维细胞作为病毒染色质的来源。选择性分离的染色质显示比成熟的Simian病毒40染色质略大（80秒），其在蔗糖密度梯度中进行染色。 Fo I和FO II都存在于牛乳头瘤病毒1型染色质中。一种快速沉淀的部分，其结构仍然未知，还含有低聚牛乳头瘤病毒1型DNA。通过原位DNA酶消化分离的核和随后的牛乳头瘤病毒1型DNA具有各种限制性内切核酸酶的DNA，在染色质中检测到主要的DNase-过敏区。包含大约320个碱基对的该区域位于相对物理地图位置0.88和0.92之间。

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