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首页> 外文期刊>Journal of Virology >rRNA cleavage as an index of ppp(A2'p)nA activity in interferon-treated encephalomyocarditis virus-infected cells.
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rRNA cleavage as an index of ppp(A2'p)nA activity in interferon-treated encephalomyocarditis virus-infected cells.

机译：RRNA切割作为干扰素治疗的脑膜炎病毒感染细胞的PPP（A2'P）NA活性的指标。

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摘要

In cell-free systems, 2-5A [ppp(A2'p)nA, n = 2 to greater than or equal to 4] activates a latent endoribonuclease, the 2-5A-dependent RNase, which cleaves rRNA in intact ribosomes into discrete and characteristic products (D. H. Wreschner et al., Nucleic Acids Res. 9:1571-1581, 1981). Here we present Northern blots which have identified the 18S or 28S origins of the cleaved products from rRNA. In addition, identical 3' termini were observed for fragments of 18S rRNA from a HeLa cell-free system incubated with 2-5A and from interferon-treated, encephalomyocarditis virus-infected HeLa cells. The previous assumption of identity of such fragments was based only on comigration on electrophoresis in agarose gels. We conclude that appropriate patterns of cleavage found in RNA isolated from intact cells are an indicator of prior 2-5A-dependent RNase activity. The assay of rRNA cleavage is relatively convenient and unambiguous. Accordingly, in the search for situations in which the 2-5A system may be active, it provides a useful alternative to the direct assay of 2-5A.
机译：在无细胞系统中，2-5A [PPP（A2'P）Na，n = 2至大于或等于4]激活潜在的内衣核酸酶，2-5A依赖性RNase，其将RRNA完整地切入离散的核糖体和特征产品（DH Wreschner等，核酸Res。9：1571-1581,1981）。在这里，我们呈现Northern印迹，其已鉴定从RRNA的切割产物的18秒或28s起源。此外，从与2-5A和干扰素治疗的干扰素治疗的HeLa细胞系统中，观察到18s RRNA的片段，观察到相同的3'末端，从而从干扰素处理的脑膜炎病毒感染的HeLa细胞。此前这种片段的身份的先前假设仅基于琼脂糖凝胶中的电泳的崩溃。我们得出结论，从完整细胞分离的RNA中发现的适当裂解模式是先前2-5A依赖性RNase活性的指标。 RRNA裂解的测定相对方便和明确。因此，在寻找2-5A系统可能有效的情况下，它提供了2-5A的直接测定的有用替代方法。

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