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首页> 外文期刊>Journal of Virology >RNAs transcribed from a 3.6-kilobase SmaI fragment of the short unique region of the herpes simplex virus type 1 genome.
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RNAs transcribed from a 3.6-kilobase SmaI fragment of the short unique region of the herpes simplex virus type 1 genome.

机译：RNA从疱疹病毒类型1基因组的短独特区域的3.6千千碱酶SMAI片段转录。

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摘要

A 3.6-kilobase (kb) SmaI subclone of the BamHI J fragment of herpes simplex virus type 1 (KOS) DNA was utilized to characterize the mRNAs transcribed from the genome segment (0.91 to 0.93 map units) that encodes glycoprotein D mRNA. RNA blotting demonstrated two major RNA species of 2.3 and 1.5 kb. 5' and 3' mapping with 32P-end-labeled DNA fragments indicated that these RNAs are a nested set, each having its own promoter and 3' terminus. Less abundant RNA species with discrete 5' ends were also observed. Precise 5' mapping and sequence data located the initiation sites and demonstrated TATA boxes, CAT boxes, and AC-rich regions in the appropriate positions. 3' mapping located a common end for both mRNAs, but the 2.3-kb mRNA was reduced in size by splicing at a point near the RNA terminus. In vitro runoff transcription experiments confirmed the location of the two promoters and showed that an uninfected cell extract initiated faithfully at both sites. Despite the similarities in DNA structure and the apparent equal efficiency of promoter utilization in vitro, the 2.3-kb mRNA appeared in the cytoplasm early (1 h) after infection, whereas the 1.5-kb mRNA was delayed until 3 h after infection.
机译：用于疱疹病毒类型1（KOS）DNA的Bamhi J片段的3.6千碱基（KB）SMAI子旋网，以表征从基因组区段（0.91至0.93映射单元）转录的MRNA，其编码糖蛋白D mRNA。 RNA印迹显示出两种主要RNA物种为2.3和1.5 kB。用32p-End标记的DNA片段的5'和3'映射表明这些RNA是嵌套集合，每个嵌套集合具有其自身的启动子和3'末端。还观察到具有离散5'末端的较少丰富的RNA种类。精确的5'映射和序列数据位于启动站点，并在适当的位置演示了塔塔盒，猫盒和富型的地区。 3'映射位于两个mRNA的共源端，但是通过在RNA末端附近的点处拼接来降低2.3kb mRNA的尺寸。体外径流转录实验证实了两个启动子的位置，并显示出在两个地点忠实发起的无感染细胞提取物。尽管DNA结构的相似性和体外启动子利用的明显平等效率，但感染后早期（1小时）出现在细胞质中的2.3-kb mRNA，而感染后1.5kb mRNA延迟直至3小时。

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