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首页> 外文期刊>Journal of bacteriology >Activation and inactivation of methanol: 2-mercaptoethanesulfonic acid methyltransferase from Methanosarcina barkeri.
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Activation and inactivation of methanol: 2-mercaptoethanesulfonic acid methyltransferase from Methanosarcina barkeri.

机译：甲醇：2-巯基乙烯磺酸甲基转移酶的活化与灭活来自甲基甲蛋白酶。

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摘要

Methanol is converted to methane by crude extracts of Methanosarcina barkeri. The first reaction involved in this process, is catalyzed by methanol:2-mercaptoethanesulfonic acid methyltransferase (EC 2.1.1.-). The methyltransferase has an optimum at pH 6.5 and is not inhibited by 2-bromoethanesulfonic acid. Pyridoxal-5'-phosphate acts as an inhibitor (Ki = 0.30 mM). The methyltransferase was tested in the presence of 2-bromoethanesulfonic acid, which inhibits the conversion of 2-(methylthio)ethanesulfonic acid to methane. The reaction is subject to activation and inactivation. Inactivation is brought about by the presence of oxygen, flavin mononucleotide, flavin adenine dinucleotide, and 2-(methylthio)ethanesulfonic acid, the product of the reaction. Activation of the system requires the presence of ATP and Mg2+ and of hydrogen. Hydrogen can be replaced by enzymatic systems, such as pyruvate dehydrogenase, which deliver free hydrogen.
机译：通过甲基喹啉甲烷的粗提取物将甲醇转化为甲烷。在该方法中涉及的第一反应通过甲醇：2-巯基乙烯磺酸甲基转移酶（EC 2.1.1.-）催化。甲基转移酶在pH6.5处具有最佳的最佳状态，并且不受2-溴乙烯磺酸抑制。吡哆醛-5'-磷酸酯用作抑制剂（ki = 0.30mm）。在2-溴乙烯磺酸存在下测试甲基转移酶，其抑制2-（甲硫基）乙磺酸与甲烷的转化。反应受激活和灭活。通过存在氧气，黄素单核苷酸，黄芩腺苷二核苷酸和2-（甲硫基）乙磺酸，反应的产物来引起灭活。系统的活化需要存在ATP和Mg2 +和氢。氢气可以用酶系统代替，例如丙酮酸脱氢酶，其提供游离氢。

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