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首页> 外文期刊>Journal of Virology >Isolation and characterization of a membrane-bound population of group B coxsackieviruses.
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Isolation and characterization of a membrane-bound population of group B coxsackieviruses.

机译：B组Coxsackeiguses膜结合群的分离与表征。

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摘要

HeLa cells infected with several group B coxsackieviruses contain a previously undetected, virus-specific ribonucleoprotein particle which we designated membrane-bound virion (MBV). MBVs of B5 virus have a pronounced polygonal appearance and are slightly smaller than virions. The particles sediment more slowly (at about 107S) and have a lower buoyant density (about 1.30). They contain 35S virion RNA; only three, and not four, capsid proteins; and at least seven additional proteins with apparent molecular weights of 21,000 to 92,000. Three of the latter proteins appear to be of host origin; the rest may be precursors of virion capsid proteins. The RNA is resistant to digestion by RNase, and EDTA treatment disrupts the particle. MBVs are infectious, although significantly less so than virions. Cells infected with MBVs produce both types of progeny, virions and MBVs. In coinfected cultures, the yield of progeny is lower than in cells infected with virions alone, suggesting interference by MBVs. Synthesis of both types can be detected within 3.5 h after infection, and synthesis continues for 24 h.
机译：用几组B Coxsackeiguses感染的Hela细胞含有先前未被发现的病毒特异性核糖蛋白颗粒，其指定膜结合的病毒素（MBV）。 B5病毒的MBV具有明显的多边形外观，略小于病毒粒子。粒子沉淀得更慢（约107s）并且具有较低的浮力密度（约1.30）。它们含有35s病毒虫RNA;只有三个，而不是四，衣壳蛋白;并且至少七个另外的蛋白质，具有21,000至92,000的表观分子量。后一种蛋白质中的三个似乎是宿主起源;其余的可能是维尔岛衣壳蛋白的前体。 RNA对RNase消化的抗蚀剂，EDTA治疗破坏了颗粒。 MBV是传染性的，虽然比病毒群体显着少。感染MBV的细胞产生两种类型的后代，病毒群和MBV。在焦化的培养物中，后代的产率低于单独感染病毒粒的细胞，表明MBV的干扰。两种类型的合成可以在感染后3.5小时内检测，合成持续24小时。

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