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首页> 外文期刊>Journal of Virology >UC-1, a new bacteriophage that uses the tonA polypeptide as its receptor.
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UC-1, a new bacteriophage that uses the tonA polypeptide as its receptor.

机译：UC-1，一种使用吨多肽作为其受体的新噬菌体。

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摘要

We characterized UC-1, a previously undescribed Escherichia coli phage. UC-1 was observed to have an icosahedral head and a long, flexible, noncontractile tail: its genome consisted of linear double-stranded DNA having a molecular weight of 34 X 10(6). The product of the tonA gene served as at least part of the receptor for UC-1. E. coli tonA strains neither plated nor adsorbed UC-1 well, tonA mutants were selected on the basis of UC-1 resistance, and ferrichrome, a siderophore which utilizes TonA as its receptor, blocked infection. Restriction analyses, DNA-DNA hybridization experiments, and guanine-plus-cytosine determinations demonstrated that UC-1 DNA was unrelated to that of other phages (T1, T5, and phi 80) which employ TonA as a receptor. Also, mutants specifically resistant to UC-1 were isolated. UC-1 may be useful as a probe for investigating TonA, which functions as a receptor for more ligands than any other membrane protein. Study of the resistant mutants may improve our understanding of how phage DNA penetrates the cell envelope.
机译：我们以先前未描述的大肠杆菌噬菌体表征UC-1。观察到UC-1具有ICosaheDral头部和长，柔韧的不可触发的尾部：其基因组由分子量为34×10（6）的线性双链DNA组成。吨基因的产物作为UC-1的至少一部分受体。大肠杆菌吨株均不镀覆的UC-1孔，在UC-1抗性的基础上选择吨突变体，并且硅胶晶体，其具有吨位作为其受体，感染障碍。限制性分析，DNA-DNA杂交实验和鸟嘌呤加胞嘧啶测定证明，UC-1 DNA与使用吨作为受体的其他噬菌体（T1，T5和PHI 80）无关。而且，分离特异性耐抗UC-1的突变体。 UC-1可用作研究吨的探针，其用作比任何其他膜蛋白更多的配体的受体。对抗性突变体的研究可以改善我们对噬菌体DNA如何穿透细胞包络的理解。

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