The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane

Iwona Wower; Richard Brimacombe

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The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane

机译：16S RNA的多个位点的定位通过用2-咪罗烷处理在大肠杆菌30S核糖体亚基中与蛋白质S7和S8交联。

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RNA-protein cross-links were introduced into E. coli 30S ribosomal subunits by reaction with 2-iminothiolane followed by a mild ultraviolet irradiation treatment. After removal of non-reacted protein and partial nuclease digestion of the cross-linked 16S RNA-protein moiety, a number of individual cross-linked complexes could be isolated and the sites of attachment of the proteins to the RNA determined. Protein S8 was cross-linked to the RNA at three different positions, within oligo-nucleotides encompassing positions 629–633, 651–654, and (tentatively) 593–597 in the 16S sequence. Protein S7 was cross-linked within two oligonucleotides encompassing positions 1238–1240, and 1377–1378. In addition, a site at position 723–724 was observed, cross-linked to protein S19, S20 or S21.

机译：通过与2-咪喹甲酸盐的反应，将RNA-蛋白质的交联剂引入大肠杆菌30S核糖体亚基中，然后用温和的紫外线照射处理。除去非反应的蛋白质和部分核酸酶消化的交联的16S RNA蛋白部分，可以分离多种单独的交联络合物，并且将蛋白质的附着位点与测定的RNA附着。在寡核苷酸内以三种不同的位置交联与RNA的蛋白质S8，包括在16S序列中的位置629-633,651-654和（暂时）593-597中的寡核苷酸。在包含位置1238-1240和1377-1378的两个寡核苷酸内交联蛋白质S7。另外，观察到位置723-724的位点，与蛋白质S19，S20或S21交联。

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《Nucleic acids research》 |1983年第5期|共19页
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Iwona Wower; Richard Brimacombe;
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收录信息美国《科学引文索引》(SCI);
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1. The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane [J] . Iwona Wower, Richard Brimacombe Nucleic acids research . 1983,第5期

机译：16S RNA的多个位点的定位通过用2-咪罗烷处理在大肠杆菌30S核糖体亚基中与蛋白质S7和S8交联。
2. The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane [J] . Iwona Wower, Richard Brimacombe Nucleic acids research . 1983,第5期

机译：16S RNA的多个位点的定位通过用2-咪罗烷处理在大肠杆菌30S核糖体亚基中与蛋白质S7和S8交联。
3. RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S5, S7, S8, S9, S11, S13, S19 and S21 by treatment with methyl p-azidophenyl acetimidate [J] . Barbara Greuer, Georg St?ffler, Hans B?umert, Nucleic acids research . 1987,第8期

机译：大肠杆菌30S核糖体亚基中的RNA-蛋白质交联;通过用对叠氮基苯基乙酰胺酸甲酯处理确定与蛋白质S3，S4，S5，S7，S8，S9，S11，S13，S19和S21交联的16S RNA上的位点
4. Using Mass Spectrometry to Identify the Components of the Erythromycin-Induced 50S Ribosomal Subunit Assembly Particle in Escherichia coli [C] . Romel P. Dator, Patrick A. Limbach American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：使用质谱法鉴定大肠杆菌中红霉素诱导的50s核糖体亚基组装颗粒的组分
5. Characterization of a 30S ribosomal subunit assembly intermediate found in Escherichia coli cells growing with neomycin or paromomycin [D] . Foster, Cerrone Renee. 2007

机译：30S核糖体亚基组装中间体的表征在大肠杆菌细胞中生长的大肠杆菌细胞中中间体或副霉素生长
6. The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane. [O] . I Wower, R Brimacombe 1983

机译：通过用2-亚氨基硫烷处理在大肠杆菌30S核糖体亚基中与蛋白质S7和S8交联的16S RNA上多个位点的定位。
7. The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane. [O] . Wower, I, Brimacombe, R 1983

机译：通过用2-亚氨基硫杂环戊烷处理，在大肠杆菌30S核糖体亚基中与蛋白质S7和S8交联的16S RNA上多个位点的定位。

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The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane

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