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首页> 外文期刊>Journal of Virology >Synthesis of multimeric polyoma virus DNA in mouse L-cells: role of the tsA1S9 gene product.
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Synthesis of multimeric polyoma virus DNA in mouse L-cells: role of the tsA1S9 gene product.

机译：小鼠L细胞中多聚体多瘤病毒DNA的合成：TSA1S9基因产物的作用。

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Several different forms of progeny viral DNA can be identified in polyoma virus (Py)-infected mouse L-cells. The majority comprise mature form I superhelical DNA and the circular, double-stranded "theta" replicating intermediates in which the progeny DNA strands never exceed the unit genome length of the template. There is formed, in addition, a minority fraction of multimeric, linear, double-stranded Py DNA molecules that sediment heterogeneously at 28 to 35S and greater than 35S. Restriction enzyme analysis of these large Py DNA molecules reveals them to be tandem arrays of multiple unit genome lengths, covalently linked head to tail. It is estimated that the 28 to 35S multimeric DNA has an average size of about 20 megadaltons, made up of 6 to 20 Py genome units. The greater than 35S Py DNA is, of course, larger. Kinetic analysis indicates that formation of the monomeric progeny viral DNA and the 28 to 35S multimeric Py DNA reaches a peak at about 35 to 36 h postinfection. Synthesis of the very large linear molecules of greater than 35S is first detected after this interval and continues thereafter. The de novo synthesis of all of these progeny Py DNA molecules proceeds apparently normally in Py-infected tsA1S9 mouse L-cells incubated at 38.5 degrees C under conditions which restrict normal cellular DNA replication. These findings suggest that the cellular DNA topoisomerase II activity, encoded in the tsA1S9 locus (R. W. Colwill and R. Sheinin, submitted for publication), is not required for de novo formation of any form of Py DNA. However, the total amount made and the rate of synthesis of the large molecular weight Py DNA are affected very late in temperature-inactivated tsA1S9 cells.
机译：可以在多瘤病毒（PY） - 培养的小鼠L细胞中鉴定几种不同形式的后代病毒DNA。大多数包括成熟形式的I超象性DNA和圆形双链“θ”复制中间体，其中后代DNA链永远不会超过模板的单位基因组长度。此外，还形成了少数群体的多聚体，线性的双链PY DNA分子，其沉积在28至35s和大于35s的28-35s中。这些大PY DNA分子的限制酶分析揭示了它们是多单位基因组长度的串联阵列，将头部与尾部共价连接。估计，28至35s的多聚体DNA的平均尺寸约为20兆塔尔顿，由6至20 py基因组单元组成。当然，大于35s的py DNA是更大的。动力学分析表明，形成单体后代病毒DNA和28至35s多聚体PY DNA的形成达到峰值的峰值。在该间隔之后首先检测到大于35s的非常大的线性分子的合成，然后继续。所有这些后代PY DNA分子的DE Novo合成显然通常在Py感染的TSA1S9小鼠L细胞中，在限制正常细胞DNA复制的条件下在38.5摄氏度下孵育。这些发现表明，在TSA1S9基因座（R.W.Co.Colwill和R.Hehinin，提交出版物的R.W.Colwill和R. Seinin）的细胞DNA拓扑异构酶II活性不需要任何形式的PY DNA。然而，大分子量Py DNA的总量和合成速率在温度灭活的TSA1S9细胞中非常晚。

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    《Journal of Virology》 |1983年第3期|共10页
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    P R Ganz; R Sheinin;

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  • 收录信息 美国《科学引文索引》(SCI);
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