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首页> 外文期刊>Journal of Virology >Synthesis of plus- and minus-strand RNA from poliovirion RNA template in vitro.
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Synthesis of plus- and minus-strand RNA from poliovirion RNA template in vitro.

机译：在体外合成来自Poliovirion RNA模板的加股和股线RNA。

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The poliovirus RNA polymerase, 3Dpol, was used to synthesize RNA in vitro in the presence of a host factor preparation from uninfected HeLa cells and poliovirion RNA as the template. The transcription products included molecules approximately twice the length of the template, apparently resulting from hairpin formation and template-directed elongation, as previously reported (D. C. Young, D. M. Tuschall, and J. B. Flanegan, J. Virol. 54:256-264, 1985). Other polyadenylated template RNAs also yielded products that were twice the length of the template. The polarity of the products synthesized from plus-strand poliovirus RNA template was analyzed by Southern blotting using labeled product RNA to probe single-stranded poliovirus DNAs cloned into M13 vectors. The results demonstrated that host factor-mediated polymerase products contain newly synthesized plus-strand sequences as well as the expected minus-strand sequences. Polymerase products primed with oligo(U) were all of minus-strand polarity.
机译：脊髓灰质炎患者RNA聚合酶3DPol，用于在宿主因子制备的存在下在宿主因子制备中合成RNA作为模板。转录产品包括分子大约是模板长度的两倍，显然是由簪形成和模板导向的伸长率（DC Young，DM Tuschall和JB Flanegan，J.Virol。54：256-264,1985） 。其他聚酰胺化模板RNA也产生了两倍的模板的两倍。通过Southern印迹通过标记产物RNA通过Southern印迹分析了从加链脊髓灰质炎模板中合成的产品的极性，以探测克隆到M13载体中的单链脊髓灰质炎DNA。结果证明，宿主因子介导的聚合酶产物含有新合成的加股序列以及预期的负链序列。用寡核苷酸（U）施加的聚合酶产物均为负链极性。

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