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首页> 外文期刊>Journal of Virology >Monoclonal antibodies to hemagglutinin-neuraminidase and fusion glycoproteins of Newcastle disease virus: relationship between glycosylation and reactivity.
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Monoclonal antibodies to hemagglutinin-neuraminidase and fusion glycoproteins of Newcastle disease virus: relationship between glycosylation and reactivity.

机译：血凝素 - 神经氨酰胺酶的单克隆抗体和融合糖蛋白的新城疫病毒：糖基化与反应性之间的关系。

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摘要

Eighteen hybridoma lines obtained by immunization of mice with Newcastle disease virus (NDV) lentogenic strain La Sota or velogenic strain Italien produced hemagglutinating monoclonal antibodies. The 18 monoclones were divided into four groups according to their reactivity toward native hemagglutinin neuraminidase protein (HN), nonglycosylated HN precursor, and heat-denatured HN blotted on nitrocellulose membranes. Only group II reagents were reactive toward their targets in all conditions tested. They were considered sequence-specific antibodies. Group I antibodies did not require glycosylation but lacked reactivity towards the denatured glycosylated antigen. Monoclonal antibodies from group III recognized only the native HN. Group IV was made up of a single monoclone that lacked reactivity with NDV Italien but recognized the La Sota strain in hemagglutination inhibition and enzyme-linked immunosorbent assays. Five hybridoma lines produced monoclonal antibodies which neutralized viral infectivity but failed to inhibit hemagglutination. One monoclonal antibody obtained after immunization of mice with NDV La Sota showed a low neutralization index versus NDV Italien. Four monoclonal antibodies derived from mice immunized with NDV Italien showed higher neutralization indices towards this strain. Neither the denatured F protein nor its nonglycosylated precursor was reacted against by the five monoclonal antibodies.
机译：通过用新城疫病毒（NDV）乙二酚诱导菌株La Sota或麝香菌株Italien产生的18条杂交瘤线产生血凝单克隆抗体。将18个单克隆根据其对天然血凝素神经氨酰胺酶蛋白（HN），尼糖基化的HN前体的反应性，并在硝酸纤维素膜上呈中的热变性HN。在测试的所有条件下，只有II族试剂对其靶标反应。它们被认为是序列特异性抗体。 I族抗体不需要糖基化但缺乏对变性糖基化抗原的反应性。来自III组的单克隆抗体仅识别天然HN。第IV组由单胶质组成，单晶单均缺乏与NDV Italien的反应性，但是血凝抑制抑制和酶联免疫吸附试验中的La Sota菌株。五种杂交瘤系产生了中和病毒感染性但未抑制血凝抗体的单克隆抗体。在用NDV La Sota免疫小鼠之后获得的一种单克隆抗体显示出低中和指数与NDV Italien。衍生自与NDV Italien免疫的小鼠的四种单克隆抗体显示出朝向该菌株的更高的中和索引。既不是变性的f蛋白质也不是其氮胶质化前体，由五种单克隆抗体反应。

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