• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Characterization of the restricted component of Epstein-Barr virus early antigens as a cytoplasmic filamentous protein.
【24h】

Characterization of the restricted component of Epstein-Barr virus early antigens as a cytoplasmic filamentous protein.

机译：Epstein-Barr病毒早期抗原作为细胞质丝状蛋白的限制成分的表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Four monoclonal antibodies produced against the restricted component of the Epstein-Barr virus (EBV) early antigen (EA-R) precipitated a polypeptide with an approximate molecular weight of 85,000. Three of these antibodies prepared against the native 85,000-molecular-weight protein (85K protein) reacted by immunofluorescence with acetone-fixed smears but not methanol-fixed smears of EBV-producing cells activated with tumor-promoting agent and sodium butyrate. The fourth monoclonal antibody which was produced against the denatured 85K protein reacted with both acetone-fixed cells and methanol-fixed cells. Blocking of direct immunofluorescence by the different monoclonal antibodies established that these monoclonal antibodies were directed against three different epitopes expressed on the 85K protein. The cytoplasmic staining pattern produced by each antibody was granular during the first 24 to 28 h after induction, developed into filamentous structures about 36 h after induction, and then began to aggregate after 48 h. Similar structures were observed in human placental cells transfected by EBV DNA and stained with three of the monoclonal antibodies. These results suggest that the EA-R polypeptide is assembled into filaments during the EBV lytic cycle. The significance of this in regards to replication has yet to be determined. Biochemical characterization of this major EA-R component did not reveal any major differences in this protein isolated from different cell lines.
机译：抵抗Epstein-Barr病毒（EBV）早期抗原（EA-R）的限制组分产生的四种单克隆抗体沉淀出多肽，其分子量为85,000。这些抗体中的三种抗体由丙酮固定涂片的免疫荧光反应，而不是用肿瘤促进剂和丁酸钠活化的EBV生产细胞的甲醇固定涂片。抵靠变性的85K蛋白质产生的第四单克隆抗体与丙酮固定细胞和甲醇固定细胞反应。通过不同单克隆抗体阻断直接免疫荧光确立了这些单克隆抗体针对在85K蛋白上表达的三种不同表位。通过每种抗体产生的细胞质染色图案在诱导后的前24至28小时内是颗粒状，在诱导后约36小时开发到丝状结构中，然后在48小时后开始骨料。在EBV DNA转染的人胎盘细胞中观察到类似的结构，并用三种单克隆抗体染色。这些结果表明EA-R多肽在EBV裂解循环期间组装成长丝。对复制的重要性尚未确定。这种主要EA-R组分的生化表征未揭示该蛋白质与不同细胞系的任何主要差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号