...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Molecular cloning and characterization of gag-, pol-, and env-related gene sequences in the ev- chicken.
【24h】

Molecular cloning and characterization of gag-, pol-, and env-related gene sequences in the ev- chicken.

机译：EV-鸡肉中胶质，酚和胚胎相关基因序列的分子克隆与表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Using less stringent hybridization conditions and cloned viral DNA probes representing the avian sarcoma virus gag, pol, env, and long terminal repeat (LTR) gene sequences, we detected related sequences in two avian species purportedly lacking all endogenous avian leukosis viruses, the ev- chicken and the Japanese quail. The blot hybridization patterns obtained with the various probes suggest the presence of between 40 and 100 copies of retrovirus-related sequences in the genomes of these two species. An ev- chicken genomic DNA library was prepared and screened with gag-specific and pol-specific DNA probes. Several different clones were obtained from this library and characterized. Analysis of these clones revealed that the retrovirus-related gene sequences are linked in the order LTR-gag-pol-env-LTR, a structure indicative of a complete provirus. These data indicate the presence of previously unidentified endogenous retrovirus species in avian cells, suggesting that under the appropriate conditions of hybridization additional, more distantly evolved families of endogenous retrovirus genes may be identified in vertebrate species.
机译：代表代表禽Sarcoma病毒Gag，Pol，Env和Long终端重复（LTR）基因序列的克隆病毒DNA探针，我们据称在据称缺乏所有内源性禽白血病病毒，EV-鸡和日本鹌鹑。用各种探针获得的印迹杂交模式表明，在这两个物种的基因组中存在40％至100拷贝的逆转录病毒相关序列。制备EV-鸡基因组DNA文库并用GAG特异性和POT特异性的DNA探针筛选。从该文库获得了几种不同的克隆，其特征。这些克隆的分析显示，逆转录病毒相关的基因序列在LTR-GAG-POL-env-LTR中链接，该结构指示完整的潜意症。这些数据表明存在先前未识别的内源性逆转录病毒在禽细胞中的存在，表明在适当的杂交条件下，可以在脊椎动物物种中鉴定更远方地进化的内源性逆转录病毒基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号