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首页> 外文期刊>Journal of Virology >Active synthesis of hemagglutinin-specific immunoglobulin A by lung cells of mice that were immunized intragastrically with inactivated influenza virus vaccine.
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Active synthesis of hemagglutinin-specific immunoglobulin A by lung cells of mice that were immunized intragastrically with inactivated influenza virus vaccine.

机译：血凝细胞的活性合成小鼠肺细胞与胃部免疫血小杂化病毒疫苗免疫的肺细胞。

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Intragastric inoculation with whole-virion vaccine of inactivated influenza virus resulted in production of hemagglutinin (HA)-specific immunoglobulin A (IgA) and IgG both in lung lavage fluids and in serum samples of mice. HA-specific IgA was the predominant isotypic antibody secreted in the lung lavage fluids (average IgA/IgG ratio, 13:1), whereas HA-specific IgG was the major antibody class in serum (average IgA/IgG ratio, 0.3:1). These responses were similar to the antibody responses stimulated by intranasal infection with live influenza virus. In vitro cultures of lymphoid cells from lungs and Peyer's patches, but not from spleens, in the presence of homologous antigen, from mice vaccinated intragastrically synthesized mostly HA-specific IgA. Mice immunized parenterally with inactivated influenza virus produced only IgG in lung lavage fluids and sera. Cultures of lymphoid cells from their spleens, but not their lungs, synthesized HA-specific IgG upon antigenic stimulation in vitro; neither synthesized IgA. These in vitro cell culture results, as well as the inverse relationship of IgA/IgG ratios in lung lavage fluids and sera, demonstrated that the IgA antibody in lung lavage fluids was actively synthesized locally in the lungs of intragastrically immunized mice. This finding was consistent with the migratory distribution of antigen-primed lymphoid cells from Peyer's patches to distant lymphoid tissue such as lung. Intragastric vaccination conferred protection against intranasal challenge with a lethal dose of virulent virus.
机译：胃内接种与灭活流感病毒的全部病毒藻疫苗导致血凝素（HA）的血凝素（HA）和IgA）和IgG在肺灌洗液中和小鼠血清样品中产生。 HA特异性IgA是在肺灌洗液中分泌的主要同种型抗体（平均IgA / IgG比，13：1），而HA特异性IgG是血清中的主要抗体类（平均IgA / IgG比，0.3：1） 。这些反应类似于通过与活流感病毒的鼻内感染刺激的抗体反应类似。来自肺和Peyer斑块的淋巴细胞的体外培养物，但不是来自脾脏，在同源抗原的存在下，从血管接种的胃地组合成大多数HA特异性IgA。用灭活的流感病毒免疫肠外免疫的小鼠仅在肺灌洗液和血清中产生IgG。淋巴细胞的培养物来自脾脏，但不是它们的肺，在体外抗原刺激后合成HA特异性IgG;既不合成IgA。这些体外细胞培养结果以及肺灌洗液和血清中IgA / IgG比的逆关系表明，肺灌洗液中的IgA抗体在胃肠免疫小鼠的肺部局部地局部地合成。该发现与抗原浸涂淋巴细胞的迁移分布从Peyer的贴片到远处淋巴组织如肺部。胃内疫苗接种赋予含有致死剂量的毒性病毒的鼻内挑战保护。

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