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首页> 外文期刊>Journal of Virology >Herpes simplex virus immediate-early promoters are responsive to virus and cell trans-acting factors.
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Herpes simplex virus immediate-early promoters are responsive to virus and cell trans-acting factors.

机译：单纯疱疹病毒立即早期启动子对病毒和细胞转运因子敏感。

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The promoters for each of the immediate-early genes from herpes simplex virus type 1 were cloned and fused to a chloramphenicol acetyltransferase cassette. These chimeric genes were used as targets in a transient expression assay to determine how the immediate-early gene products ICP4 and ICP0 and the virion-associated stimulatory protein Vmw65 affected their expression in HeLa and Vero cells. The basal level of expression from these cassettes differed significantly depending on the extent of 5'-flanking sequence and the cell line that served as host. The promoters from IE-4 and IE-0 behaved in a qualitatively similar fashion independent of the host cell. However, the promoter for ICP27 had a unique response pattern: in Vero cells it acted as an alpha gene promoter, whereas in HeLa cells its response was more like that of a beta gene promoter. The promoter sequences for ICP22 and ICP47 behaved as the IE-4 and IE-0 promoters did in HeLa cells, but their response to the effector molecules in Vero cells was unlike that of other alpha gene promoters we have studied. Evidence is also presented for a role for ICP27 in autoregulation.
机译：将来自疱疹病毒类型1的每个直接早期基因的启动子被克隆并融合给氯霉素乙酰转移酶盒。将这些嵌合基因用作瞬时表达测定中的靶标，以确定近期早期基因产物ICP4和ICP0以及病毒患者相关的刺激蛋白VMW65影响其在HeLa和Vero细胞中的表达。这些盒的基础表达水平显着不同，这取决于5'侧面序列和作为宿主的细胞系的程度不同。来自IE-4和IE-0的启动子以与宿主细胞无关的定性类似的方式。然而，ICP27的启动子具有独特的响应模式：在Vero细胞中，它充当α基因启动子，而在HeLa细胞中，其响应更像是β基因启动子的响应。 ICP22和ICP47的启动子序列表现为IE-4和IE-0启动子在HeLa细胞中表现，但它们对Vero细胞中的效应分子的反应与我们研究的其他α基因启动子的反应不同。还呈现了ICP27在自动调节中的作用。

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