Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus.

P D Friesen; L K Miller

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Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus.

机译：由杆菌病毒综述Californica核多面体病毒的HindIII k基因组片段编码的35- kindalton蛋白基因的发散转录。

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The organization of viral genes within the 3.7-kilobase-pair HindIII-K/EcoRI-S region of the Autographa californica nuclear polyhedrosis virus genome (85 to 88 map units) was determined by using a combination of nucleotide sequencing, transcriptional mapping, and in vitro translation of hybrid selected RNA. Two nonoverlapping genes, extending in opposite directions and encoding polypeptides with molecular weights of 35,000 and 94,000 (35K and 94K polypeptides), were identified. Unspliced, messenger-active RNAs were transcribed from both genes early (2 h) after infection. Indicative of immediate-early genes, transcription of the divergent RNAs was unaffected by the protein synthesis inhibitor, cycloheximide. Late in infection, abundant RNAs were transcribed from promoters located at least 2.5 kilobase pairs upstream from the gene encoding the 35K polypeptide. These transcripts completely overlapped both the 35K and 94K polypeptide genes but apparently lacked protein-coding potential, suggesting that the transcripts may play a role in the suppression of early viral gene expression.

机译：通过使用核苷酸测序，转录测绘的组合，通过使用核苷酸测序，转录映射和进入的组合来确定在3.7千碱基 - 对核多面体病毒基因组（85至88个地图单元）内的病毒基因组织。杂交选择的杂种术译。鉴定了两个非植入基因，沿相反方向延伸并编码具有35,000和94,000（35K和94K多肽）的分子量的多肽。感染后早期（2小时）的两种基因转录，杂散活性的RNA被转录。表示立即早期基因，发散RNA的转录不受蛋白质合成抑制剂环己酰亚胺的影响。在感染后期，从位于编码35K多肽的基因上游至少2.5千碱基对的启动子转录丰富的RNA。这些转录物完全重叠了35K和94K多肽基因，但显然缺乏蛋白质编码势，表明转录物可能在抑制早期病毒基因表达中发挥作用。

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《Journal of Virology》 |1987年第7期|共9页
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P D Friesen; L K Miller;
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1. Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus. [J] . P D Friesen, L K Miller Journal of Virology . 1987,第7期

机译：由杆菌病毒综述Californica核多面体病毒的HindIII k基因组片段编码的35- kindalton蛋白基因的发散转录。
2. Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus. [J] . P D Friesen, L K Miller Journal of Virology . 1987,第7期

机译：由杆菌病毒综述Californica核多面体病毒的HindIII k基因组片段编码的35- kindalton蛋白基因的发散转录。
3. Identification and characterization of a protein kinase-interacting protein encoded by the Autographa californica nuclear polyhedrosis virus. [J] . Fan X, McLachlin JR, Weaver RF Virology . 1998,第2期

机译：加利福尼亚州Autographa californica核多角体病毒编码的蛋白激酶相互作用蛋白的鉴定和表征。
4. Mining Heat Stress Induced Genes Encoding Heat Shock Proteins (HSPs) and Heat Stress Transcription Factors (HSFs) in Strawberry (Fragaria x ananassa) [C] . Lee-Fong Lin, Shih-Yi Liao, Jui-Hung Yen, International Conference on Mechanics and Control Engineering . 2014

机译：采矿热应激诱导在草莓（Fragaria x ananassa）中编码热休克蛋白（HSP）和热应激转录因子（HSF）的热应激诱导基因
5. Identification and characterization of the IE-N gene from the Autographa californica nuclear polyhedrosis virus [D] . Carson, David Dean 1990

机译：加利福尼亚州的Autographa californica核多角体病毒的IE-N基因的鉴定和表征
6. Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus. [O] . P D Friesen, L K Miller 1987

机译：由杆状病毒Autographa californica核多角体病毒的HindIII K基因组片段编码的早期35-和94-kilodalton蛋白基因的不同转录。
7. Nucleotide sequence and transcriptional analysis of a gene encoding gp41, a structural glycoprotein of the baculovirus Autographa californica nuclear polyhedrosis virus [O] . M Whitford, P Faulkner 1992

机译：编码GP41的基因的核苷酸序列和转录分析，杆状病毒综述Californica核多角病毒的结构糖蛋白
8. In vitro Competition of Natural 'Autographa Californica' Nuclear Polyhedrosis Virus and a Recombinant Expressing a Polyhedrin-Beta Galactosidase Fusion Protein [R] . Kawanishi, C. Y. , Huang, Y. S. , Bobseine, K. L. , 1988

机译：天然'苜蓿银纹夜蛾核型多角体病毒与表达多角体蛋白-β半乳糖苷酶融合蛋白的重组体外竞争

Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus.

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