Localization of Escherichia coli RNA polymerase-binding sites on bacteriophage S13 replicative form I DNA by protection of restriction enzyme cleavage sites.

M Ringuette; J H Spencer

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Localization of Escherichia coli RNA polymerase-binding sites on bacteriophage S13 replicative form I DNA by protection of restriction enzyme cleavage sites.

机译：通过保护限制酶切割位点，对噬菌体S13的大肠杆菌RNA聚合酶结合位点的定位。

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Protection of restriction endonuclease cleavage sites by Escherichia coli RNA polymerase bound to the replicative form I of bacteriophage S13 DNA has been used to identify a number of regions of RNA polymerase binding. Digestion with HincII, AluI, HinfI, or HaeIII, under conditions optimized for "open" complex formation, revealed 12 regions of RNA polymerase binding. Based on differential salt sensitivities, five of the regions were classified as strong or tight binding sites. These were located before genes A (two sites), B, and D and at the 5' end of gene F. The seven regions which exhibited weaker binding were located at the 5' end of gene C (two sites), in the middle of gene D, just before and at the 3' end of gene F, at the 5' end of gene G, and in the middle of gene H. The sites before genes B and D coincide with sites previously identified as promoters in bacteriophage phi X174. One of the sites before gene A, that at nucleotides 5175-5211, represents a new putative promoter site in bacteriophage S13 and phi X174 located before the previously identified A gene promoter at nucleotides 10-45.

机译：通过对噬菌体S13 DNA的复制形式I结合的大肠杆菌RNA聚合酶保护限制性内切核酸酶切割位点用于鉴定RNA聚合酶结合的许多区域。用Hincii，Alui，Hinfi或Haeiii消化，在优化为“开放”复杂形成的条件下，揭示了12个RNA聚合酶结合区域。基于差异盐敏感性，其中五个区域被归类为强或紧密的结合位点。这些位于基因A（两个位点），B和D以及在基因F的5'末端之前。在中间的5'末端表现出较弱的结合的七个区域基因D，在基因F的3'结束之前和在基因G的5'末端，在基因H的中间，在B和D基因之前的位点与先前被鉴定为噬菌体PHI中的启动子的部位x174。基因A之前的一个位点，即在核苷酸5175-5211，表示在核苷酸10-45处先前鉴定的基因启动子之前的噬菌体S13和PHI X174中的新推定启动子位点。

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《Journal of Virology》 |1987年第7期|共7页
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M Ringuette; J H Spencer;
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1. Localization of Escherichia coli RNA polymerase-binding sites on bacteriophage S13 replicative form I DNA by protection of restriction enzyme cleavage sites. [J] . M Ringuette, J H Spencer Journal of Virology . 1987,第7期

机译：通过保护限制酶切割位点，对噬菌体S13的大肠杆菌RNA聚合酶结合位点的定位。
2. Localization of Escherichia coli RNA polymerase-binding sites on bacteriophage S13 replicative form I DNA by protection of restriction enzyme cleavage sites. [J] . M Ringuette, J H Spencer Journal of Virology . 1987,第7期

机译：通过保护限制酶切割位点，对噬菌体S13的大肠杆菌RNA聚合酶结合位点的定位。
3. The GTP-Dependent Restriction Enzyme McrBC from Escherichia coli Forms High-Molecular Mass Complexes with DNA and Produces a Cleavage Pattern with a Characteristic 10-Base Pair Repeat [J] . Uwe Pieper, Detlef H. Groll, Sandra Wunsch, Biochemistry . 2002,第16期

机译：来自大肠杆菌的GTP依赖性限制性内切酶McrBC形成具有DNA的高分子量复合物，并产生具有特征性10碱基对重复的切割模式
4. DNA looping and cleavage by restriction enzymes studied by manipulation of single DNA molecules with optical tweezers [C] . Douglas E. Smith, Gregory J. Gemmen, Rachel Millin Optical Trapping and Optical Micromanipulation III . 2006

机译：用光镊操作单个DNA分子研究限制酶对DNA的环化和切割
5. Functional organization of the Escherichia coli RecA protein: Alteration of the nucleoside triphosphate catalytic domain, indentification of single-stranded DNA binding site(s), and cleavage of the LexA repressor. [D] . Rehrauer, William Michael. 1996

机译：大肠杆菌RecA蛋白的功能组织：核苷三磷酸催化结构域的改变，单链DNA结合位点的鉴定以及LexA阻遏物的裂解。
6. Localization of Escherichia coli RNA polymerase-binding sites on bacteriophage S13 replicative form I DNA by protection of restriction enzyme cleavage sites. [O] . M Ringuette, J H Spencer 1987

机译：通过限制酶切位点的保护将大肠杆菌RNA聚合酶结合位点定位在噬菌体S13复制型I DNA上。
7. Localization of Escherichia coli RNA polymerase-binding sites on bacteriophage S13 replicative form I DNA by protection of restriction enzyme cleavage sites [O] . M Ringuette, J H Spencer 1987

机译：通过保护限制酶切割位点，对噬菌体S13的大肠杆菌RNA聚合酶结合位点的定位通过保护酶切割位点

Localization of Escherichia coli RNA polymerase-binding sites on bacteriophage S13 replicative form I DNA by protection of restriction enzyme cleavage sites.

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