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首页> 外文期刊>Journal of Virology >Regulation of transcription of the Xp10 genome in bacteriophage-infected Xanthomonas campestris pv. oryzae.
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Regulation of transcription of the Xp10 genome in bacteriophage-infected Xanthomonas campestris pv. oryzae.

机译：XP10基因组在噬菌体感染Xanthomonas Campestris PV中的转录调节。 oryzae。

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Results of in vivo studies showed that the transcription of the Xp10 genome in Xp10-infected cells shifted from rifampin sensitivity to rifampin resistance. Results of in vitro studies showed that a rapid reduction of rifampin-sensitive RNA polymerase activity coincided with a rapid increase of rifampin-resistant RNA polymerase activity in cell extracts with time after infection. Host and Xp10-encoded RNA polymerases were purified, and the transcripts from these two enzymes were hybridized to the restriction fragments of Xp10 DNA. The RNA probe generated by host RNA polymerase hybridized strongly to the leftmost 25% of Xp10 DNA and weakly to the rightmost 75% of Xp10 DNA. The RNA probe generated by Xp10 RNA polymerase hybridized strongly to the rightmost 75% of Xp10 DNA and weakly to the leftmost 25% of Xp10 DNA. Studies with 32P-labeled RNA isolated at various intervals after infection did not reveal any evidence for early versus late differences in transcription.
机译：体内研究的结果表明，XP10基因组在XP10感染细胞中的转录从利福平敏感性转移到利福平抗性。体外研究的结果表明，利福平敏感性RNA聚合酶的快速降低恰好在感染后的时间随时间的时间快速增加了细胞提取物中的利福平抗性RNA聚合酶活性。纯化宿主和XP10编码的RNA聚合酶，并将来自这两种酶的转录物杂交至XP10 DNA的限制片段。通过宿主RNA聚合酶产生的RNA探针强烈地杂交至最左侧的25％的XP10 DNA，弱到最右侧75％的XP10 DNA。 XP10 RNA聚合酶产生的RNA探针强烈地杂交至最右侧75％的XP10 DNA，弱到最左侧的XP10 DNA的25％。在感染后以各个间隔分离32P标记的RNA的研究没有揭示早期与转录后期差异的任何证据。

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