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首页> 外文期刊>Vitis >Analysis of the transcript levels of VvAdhl, VvAdh2 and VvGrip4, three genes highly expressed during Vitis vinifera L. berry development
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Analysis of the transcript levels of VvAdhl, VvAdh2 and VvGrip4, three genes highly expressed during Vitis vinifera L. berry development

机译：VvAdhl，VvAdh2和VvGrip4的转录本水平分析，这三个基因在葡萄（Vitis vinifera L.berry）发育过程中高度表达

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摘要

Well defined promoter sequences are required for the targeted expression of genes in transgenic grapevines. This paper describes a detailed study of the expression of three genes with potentially useful promoters. We have used real-time RT-PCR to evaluate the transcript levels of VvAdhl, VvAdh2 and VvGrip4 genes in various tissues (root, bud, tendril, inflorescence, fruit and embryogenic callus) of Vitis vinifera L. at different stages of development. Transcript levels of the three genes were highest in berry tissues but each had a distinct pattern of expression. VvAdhl showed higher transcript levels during the early stages of berry development, while levels of VvAdh2 and VvGrip4 were higher during ripening. However, none of these genes was expressed in a strictly fruit-specific manner. In particular, significant levels of VvAdhl and VvGrip4 transcripts were observed during late tendril development and in the inflorescence, respectively. Transcript levels of all three genes were similar in both flesh and skin, indicating no tissue-specificity within the berry. Promoter sequences of the VvAdhl, VvAdh2 and VvGrip4 genes could be very useful to drive ectopic gene expression in berries of transgenic plants.
机译：在转基因葡萄中靶向表达基因需要良好定义的启动子序列。本文描述了三个具有潜在有用启动子的基因表达的详细研究。我们已经使用实时RT-PCR评估了葡萄在不同发育阶段的各种组织（根，芽，卷须，花序，果实和胚性愈伤组织）中VvAdhl，VvAdh2和VvGrip4基因的转录水平。这三个基因的转录水平在浆果组织中最高，但每个都有不同的表达模式。在浆果发育的早期，VvAdhl显示出更高的转录水平，而在成熟期间，VvAdh2和VvGrip4的水平更高。但是，这些基因均未以严格的果实特异性方式表达。特别是，在卷须发育后期和花序分别观察到显着水平的VvAdhl和VvGrip4转录物。果肉和皮肤中所有三个基因的转录水平相似，表明浆果内部没有组织特异性。 VvAdhl，VvAdh2和VvGrip4基因的启动子序列对于驱动转基因植物浆果中的异位基因表达非常有用。

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