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Elicitor-induced resveratrol production in cell cultures of different grape genotypes (Vitis spp.)

机译:在不同葡萄基因型的细胞培养物中诱导诱导白藜芦醇的产生(葡萄属)

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摘要

Higher plants synthesize secondary metabolites in response to abiotic and biotic stress. Liquid cell culture may be a useful tool for studying phytoalexin biosynthesis during plant interaction with pathogens. Dime-thyl-β-cyclodextrin (DIMEB), an oligosaccharide consisting of 2,6-methylated cyclic α(1→4)-linked glucop-yranose moieties, was shown to be capable of inducing stilbene biosynthesis in liquid grape cell cultures, also in the absence of pathogenic organisms. DIMEB is capable of inducing a response in the liquid cell cultures of the Vitis vinifera cultivars Pinot Noir and Merzling and furthermore in V. amurensis and a cross between V. riparia and V. berlandieri. More detailed attention was focused on different responses in cell suspensions of Pinot Noir and the cross of V. riparia and V. berlandieri after DIMEB treatment.
机译:高等植物响应非生物和生物胁迫而合成次生代谢产物。液体细胞培养可能是研究植物与病原体相互作用过程中植物抗毒素生物合成的有用工具。 Dime-thyl-β-cyclodextrin(DIMEB)是一种由2,6-甲基化的环状α(1→4)连接的葡萄糖-吡喃糖部分组成的寡糖,能够在液态葡萄细胞培养物中诱导二苯乙烯生物合成。在没有致病菌的情况下。 DIMEB能够在葡萄品种Pinot Noir和Merzling的液体细胞培养物中以及在V. amurensis以及V. riparia和V. berlandieri之间的杂交中诱导反应。 DIMEB处理后,黑皮诺细胞悬液和河西葡萄和西伯利亚葡萄的杂交液的不同反应更加重要。

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