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首页> 外文期刊>Vitis >Somatic embryogenesis of Vitis vinifera L. (cv. Sugraone) from stigma and style culture
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Somatic embryogenesis of Vitis vinifera L. (cv. Sugraone) from stigma and style culture

机译：柱头和花柱培养的葡萄（Sv。Sugraone）体细胞胚发生

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摘要

Somatic embryo and plant regeneration were induced from stigma and style culture of grapevine (Vitis vinifera L. cv. Sugraone). To obtain somatic embryogenesis, explants were cultured on Nitsch and Nitsch basal medium (NN) supplemented with 88 mM sucrose and various combinations of the auxin beta-naphthoxyacetic acid (NO A, 0-10 pM) and the cytokinin 6-benzylaminopurine (BA, 0-9 pM). Growth regulators (BA and NO A) in the culture medium were essential for induction of somatic embryogenesis since explants incubated on hormone-free medium never regenerated somatic embryos. Usually, the regenerated somatic embryos become visible as small white globular structures on the surface of the callus 3-4 months after culture initiation. The best embryogenic response (27 %) was obtained when stigma and style explants were cultured on NN medium supplemented with 5 mu M NOA and 9 mu M BA. Somatic embryos developed into plantlets when transferred to a hormone-free semisolid NN medium. 35 % of primary somatic embryos showed secondary embryogenesis.
机译：葡萄的柱头和样式培养（Vitis vinifera L. cv。Sugraone）诱导体细胞胚和植物再生。为了获得体细胞胚发生，在补充了88 mM蔗糖的Nitsch和Nitsch基础培养基（NN）和生长素β-萘氧基乙酸（NO A，0-10 pM）和细胞分裂素6-苄基氨基嘌呤（BA， 0-9 pM）。培养基中的生长调节剂（BA和NO A）对于诱导体细胞胚发生至关重要，因为在无激素培养基上培养的外植体从未再生过体细胞胚。通常，培养开始后3-4个月，再生的体细胞胚在愈伤组织的表面上可见为小的白色球状结构。将柱头和样式外植体在补充有5μMNOA和9μMBA的NN培养基上培养时，可获得最佳的胚胎发生反应（27％）。当将体细胞胚转移到无激素的半固体NN培养基中时，会发育成小植株。 35％的原代体细胞胚显示出继发胚发生。

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