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首页> 外文期刊>Vitis >Molecular characterisation of grapevine yellows associated phytoplasmas of the stolbur-group based on RFLP-analysis of non-ribosomal DNA
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Molecular characterisation of grapevine yellows associated phytoplasmas of the stolbur-group based on RFLP-analysis of non-ribosomal DNA

机译：基于RFLP分析的非核糖体DNA的葡萄黄相关的斯托尔伯群质体的分子表征

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摘要

Grapevines, alternative host plants, and vectors from different viticultural areas of Germany were surveyed for the presence of stolbur-group phytoplasmas that are associated with grapevine yellows. Isolates from these field samples and periwinkle isolates of stolbur were characterised by RFLP-analysis of non-ribosomal DNA fragments using a combination of 6 polymorphic primer-enzyme combinations. Three of the 5 different restriction profiles that could be distinguished were found in grapevine and other field samples of plants and insects. A previously unknown isolate was detected in grapevine, vectors and the newly identified herbaceous host Calystegia sepium. The further analysis of the phylogenetic relationship between the 5 RFLP groups revealed aclose relationship between two of the isolates associated with grapevine yellows which were also found in the two Convolvulaceae Convolvulus arvensis and Calystegia sepium. The third isolate which was detected in grapevine and stinging nettle Urtica dioica is more closely related to a periwinkle isolate obtained from Lavandula officinalis than to the other two grapevine yellows isolates. Based on the data of the field survey a specific association of the three isolates to different alternative host plants is discussed.
机译：调查了德国不同葡萄栽培地区的葡萄藤，替代寄主植物和载体中是否存在与葡萄黄相关的茎茎类植物质原体。使用6种多态引物-酶组合的组合，通过RFLP分析非核糖体DNA片段，表征了这些田间样品的分离株和Stolbur的长春花分离株。在葡萄和其他植物和昆虫的野外样品中发现了5种不同的限制性限制中的三种。在葡萄藤，载体和新鉴定的草本寄主Calystegia sepium中检测到以前未知的分离株。对5个RFLP组之间的系统发生关系的进一步分析显示，与葡萄黄相关的两个分离株之间也存在密切的关系，这两个葡萄球菌中的旋花植物和旋花藤都存在。在葡萄树和荨麻荨麻中检出的第三个分离株与从熏衣草获得的长春花分离株关系密切，而与其他两个葡萄黄色分离株关系更密切。基于现场调查的数据，讨论了三种分离株与不同的替代寄主植物的特定关联。

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  • 来源
    《Vitis》 |2004年第4期|p.191-199|共9页
  • 作者

    M. LANGER; M. MAIXNER;

  • 作者单位

    Federal Biological Research Centre for Agriculture and Forestry (BBA), Institute for Plant Protection in Viticulture, Bernkastel-Kues, Deutschland;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 园艺;
  • 关键词

    Grapevine yellows; vector; phytoplasma; epidemiology;

    机译：葡萄黄;载体;植物原浆;流行病学;

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