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Chloroplast SSR markers to assess DNA diversity in wild and cultivated grapevines

机译:叶绿体SSR标记可评估野生和栽培葡萄的DNA多样性

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摘要

Simple sequence repeats (SSRs) are abundant and well-distributed throughout the nuclear genomes of Vitis vinifera L. Simple sequences length polymorphism (SSLP), caused by variation in the number of repeat units, can be detected easily by PCR using pairs of primers designed from unique sequences bordering the SSR motifs. In the last years SSLP analysis was used for cultivar genotyping and for defining genetic relationships among different varieties. Recently simple sequence repeats of several plant species were found in chloroplast DNA (PROVAN et al. 1999; BRYAN et al. 1999). CpSSRs result a powerful tool for many aspects of evolutionary population biology (PROVAN et al. 2001), including population histories (ECHT et al. 1998) and the level of differentiation (McCAULEY 1995) because or-ganelle genomes are typically non-recombinant, uniparen-tally inherited and effectively haploid. Universal angiosperm chloroplast microsatellite primers were developed by WEISING and GARDNER (1999) on the bordering sequences of 10 cpSSRs. In this study 8 of these primer pairs were tested on wild and cultivated grapevines and the degree of variation was evaluated.
机译:简单的重复序列(SSR)丰富且分布在葡萄(Vitis vinifera L)的整个核基因组中。由重复单元数量变化引起的简单的序列长度多态性(SSLP)可以通过使用设计的引物对进行PCR轻松检测来自与SSR主题接壤的独特序列。在过去的几年中,SSLP分析被用于品种的基因分型和定义不同品种之间的遗传关系。最近在叶绿体DNA中发现了几种植物物种的简单序列重复序列(PROVAN等,1999; BRYAN等,1999)。 CpSSRs为进化种群生物学的许多方面(PROVAN等,2001),包括种群历史(ECHT等,1998)和分化水平(McCAULEY 1995)提供了强大的工具,因为or-ganelle基因组通常是非重组的,单亲遗传和有效的单倍体。通用被子植物叶绿体微卫星引物由WEISING和GARDNER(1999)在10 cpSSRs的邻接序列上开发。在这项研究中,在野生和栽培葡萄上测试了其中8对引物,并评估了变异程度。

著录项

  • 来源
    《Vitis》 |2002年第3期|p.157-158|共2页
  • 作者单位

    Department of Biology, University of Milan, Milano, Italia;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 园艺;
  • 关键词

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