Extraction of DNA from eggs of grape phylloxera (Daktulosphaira vitifoliae FITCH) for use in RAPD testing

HONG LIN; M.A. WALKER

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Extraction of DNA from eggs of grape phylloxera (Daktulosphaira vitifoliae FITCH) for use in RAPD testing

机译：从葡萄根瘤菌（Daktulosphaira vitifoliae FITCH）的卵中提取DNA，用于RAPD检测

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Three different extraction methods for preparing DNA from single grape phylloxera (Daktulosphaira vitifoliae) eggs for use in PCR-RAPD assay were compared. Pulverization in a glass micro-grinder produced much more clear and consistent RAPD results than when the egg DNA was extracted with a Chelex lysis or a proteinase-K~+ lysis. Multiple single egg extractions from the same parthenogenetic colony were done with all three methods; no RAPD assay differences were detected. This technique will allow evaluation of the roles population diversity, mutation rates, and reproductive mechanisms play in the V/fw/phylloxera interaction.

机译：比较了三种不同的提取方法，这些提取方法用于从单个葡萄根腐病菌（Daktulosphaira vitifoliae）卵中制备DNA，用于PCR-RAPD分析。与使用Chelex裂解法或蛋白酶K +裂解法提取卵DNA相比，在玻璃微粉碎机中进行粉碎产生的RAPD结果更加清晰一致。使用这三种方法从同一个孤雌生殖菌落中多次提取单个卵。未检测到RAPD分析差异。该技术将允许评估种群多样性，突变率和生殖机制在V / fw /根瘤蚜虫相互作用中的作用。

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来源
《Vitis》 |1996年第2期|p.87-89|共3页
作者
HONG LIN; M.A. WALKER;
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作者单位

Department of Viticulture and Enology, University of California, Davis, USA;

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收录信息美国《科学引文索引》(SCI);
原文格式 PDF
正文语种 eng
中图分类园艺;
关键词
Vitis; DNA extraction; RAPD; phylloxera; Daktulosphaira vitifoliae; parthenogenesis;

机译：葡萄;DNA提取;RAPD;根瘤菌;白僵菌;单性生殖;

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专利

1. Analysis of genetic variation within clonal lineages of grape phylloxera (Daktulosphaira vitifoliae Fitch) using AFLP fingerprinting and DNA sequencing [J] . Vorwerk S., Forneck A. Genome . 2007,第7期

机译：利用AFLP指纹图谱和DNA测序技术分析葡萄根瘤菌（Daktulosphaira vitifoliae Fitch）克隆谱系内的遗传变异
2. Analysis of genetic variation within clonal lineages of grape phylloxera (Daktulosphaira vitifoliae Fitch) using AFLP fingerprinting and DNA sequencing [J] . Vorwerk S, Forneck A Genome . 2007,第7期

机译：利用AFLP指纹图谱和DNA测序分析葡萄根瘤菌（Daktulosphaira vitifoliae Fitch）克隆谱系内的遗传变异
3. Grape root as habitat: overwintering and population dynamics of grape phylloxera (Daktulosphaira vitifoliae Fitch) in temperate climate viticulture [J] . Hoffmann M., Ruehl E. H., Eisenbeis G., Australian Journal of Grape and Wine Research . 2016,第2期

机译：以葡萄根为生境：温带气候葡萄栽培中的葡萄根过冬和种群动态
4. Investigations on the occurrence of grape phylloxera (Viteus vitifoliae) in Austrian viticulture [C] . H Reisenzein Symposium of the British Crop Protection Council . 2005

机译：奥地利葡萄栽培葡萄植物（Viteus Vitifoliae）的调查
5. Grape phylloxera, Daktulosphaira vitifoliae (Fitch) seasonal biology, predictive model, and management in the Ozarks. [D] . Sleezer, Sandra. 2009

机译：Ozarks的葡萄根腐病（Daktulosphaira vitifoliae（Fitch）季节性生物学，预测模型和管理）。
6. The salivary gland proteome of root-galling grape phylloxera (Daktulosphaira vitifoliae Fitch) feeding on Vitis spp. [O] . Markus W. Eitle, James C. Carolan, Michaela Griesser, 2019

机译：生物凝血葡萄植物（Daktululashaira Vitifoliae Fitch）饲喂血液血糖蛋白组的唾液腺蛋白质组。
7. Controle químico da forma galícola da filoxera Daktulosphaira vitifoliae (Fitch, 1856) (Hemiptera: Phylloxeridae) na cultura da videira Chemical control of grape phylloxera Daktulosphaira vitifoliae (Fitch, 1856) leaf form (Hemiptera: Phylloxeridae) on vineyards [O] . Marcos Botton, Rudiney Ringenberg, Odimar Zanuzo Zanardi 2004

机译：在葡萄栽培中化学控制叶状鳞茎Daktulosphaira vitifoliae（Fitch，1856）（半翅目：Phylloxeridae）的化学控制在葡萄园上对葡萄状叶鳞茎Daktulosphaira vitifoliae（Fitch，1856）叶状（Hemiptera：Phyllxeridae）的化学控制。

Extraction of DNA from eggs of grape phylloxera (Daktulosphaira vitifoliae FITCH) for use in RAPD testing

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