• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vitis >Somatic embryogenesis from leaves of Vitis x Muscadinia hybrids
【24h】

Somatic embryogenesis from leaves of Vitis x Muscadinia hybrids

机译：Vitis x Muscadinia杂种叶片的体细胞胚发生

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The regeneration of the grape was achieved using organogenesis or embryogenesis from various types of explants including stems, leaves, petioles, ovules, anthers (GRAY and MEREDITH 1992) and recently from protoplasts (REUSTLE et al. 1994) but, like most woody species, the grape is still considered as recalcitrant to regeneration because procedures are restricted to only few varieties. Despite of the importance for grape improvement (OLMO 1986), there are very few results about regeneration of Muscadinia rotundifolia and its hybrids with Vitis vinifera. So far, regeneration of the muscadine grape was achieved by somatic embryogenesis from leaves (RoeACKER 1993) and zygotic embryos (GRAY 1992) but not by organogenesis. Previously, we reported a methodfor high-frequency adventitious bud formation and plant regeneration from leaves of Vitis x Muscadinia hybrids (TORREGROSA 1994). But, all attempts to induce somatic embryogenesis by anther culture have been unsuccessful (TORREGROSA and BOUQUET, unpubl.). Using the procedures described by STAMP and MEREDITH (1988) for V. vinifera, we induced somatic embryogenesis from zygotic embryos excised from mature open pollinated seeds of these hybrids. But, this has many disadvantages with respect to the genetic characterization of the regenerated plants. Here we report the possibility to induce somatic embryogenesis and regenerate plants from leaves of Vitis x Muscadinia hybrids.
机译：葡萄的再生是通过器官发生或胚胎发生从各种类型的外植体，包括茎，叶，叶柄，胚珠，花药（GRAY和MEREDITH 1992）以及最近从原生质体（REUSTLE等，1994）中实现的，但与大多数木本植物一样，该葡萄仍被认为是再生的顽固剂，因为其程序仅限于少数几个品种。尽管对葡萄改良非常重要（OLMO 1986），但关于圆叶穆斯卡丁香及其与葡萄（Vitis vinifera）的杂交的再生结果很少。到目前为止，麝香葡萄的再生是通过叶片（RoeACKER 1993）和合子胚（GRAY 1992）的体细胞胚发生而不是器官发生来实现的。以前，我们报道了一种从葡萄（Vitis x Muscadinia）杂种的叶片中高频​​不定芽形成和植株再生的方法（TORREGROSA 1994）。但是，所有通过花药培养诱导体细胞胚发生的尝试均未成功（TORREGROSA和BOUQUET，未发表）。使用STAMP和MEREDITH（1988）所描述的针对酿酒葡萄的方法，我们从这些杂种的成熟开放授粉种子中切除的合子胚诱导了体细胞胚发生。但是，这在再生植物的遗传表征方面具有许多缺点。在这里，我们报道了从葡萄树×穆斯卡丁尼亚杂种的叶片中诱导体细胞胚发生和再生植物的可能性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号