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首页> 外文期刊>In Vitro Cellular & Developmental Biology - Animal >Enrichment of epidermal stem cells of rats by Vario magnetic activated cell sorting system
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Enrichment of epidermal stem cells of rats by Vario magnetic activated cell sorting system

机译：Vario磁活化细胞分选系统富集大鼠表皮干细胞

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摘要

Epidermal stem cells (ESCs) play an important role in skin homeostasis, wound repair, and tumorigensis which have great potential in scientific research and clinical application. So, the efficient isolation of these infrequent stem cells is very important for researchers to solve the problem of low purity and insufficient quantity of stem cells in vitro. The aim of this study was to investigate a method for the enrichment of ESCs by magnetic activated cell sorting system. The isolation strategy was CD71 depletion followed by α6-integrin positive selection. The percentage of α6briCD71dim cells in isolated cells was 94.59%. Transmission electron microscopy results revealed that α6bri CD71dim cells exhibited some typical characteristics like progenitor cells, such as big nucleus, obvious nucleolus, large nuclear–cytoplasm ratio, and few organelles in cytoplasm. When cultured in vitro, the α6briCD71dim cells had greater proliferating potential and higher colony-forming ability, and high levels of epidermal stem cell markers were expressed in our positive cells. ESCs have been successfully isolated from neonatal epidermis using Vario MACS and cultured in vitro. This isolation method is simple, fast, and inexpensive, providing an important tool for tissue engineering and cell transplantation studies.
机译：表皮干细胞（ESC）在皮肤稳态，伤口修复和肿瘤发生中起着重要作用，在科学研究和临床应用中具有巨大潜力。因此，有效分离这些罕见的干细胞对于研究人员解决体外干细胞纯度低和数量不足的问题非常重要。这项研究的目的是研究一种通过磁性激活细胞分选系统富集ESC的方法。分离策略是CD71耗竭，然后进行α6-整合素阳性选择。分离细胞中α6briCD71dim细胞的百分比为94.59％。透射电镜结果显示，α6briCD71dim细胞具有一些典型特征，如祖细胞，如大细胞核，明显的核仁，大的核质比和细胞质中的细胞器少。体外培养时，α6briCD71dim细胞具有更大的增殖潜能和更高的集落形成能力，并且在我们的阳性细胞中表达了高水平的表皮干细胞标记。使用Vario MACS已成功地从新生儿表皮中分离出ESC，并进行了体外培养。这种分离方法简单，快速且廉价，为组织工程和细胞移植研究提供了重要工具。

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