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首页> 外文期刊>In Vitro Cellular & Developmental Biology - Animal >The primary culture of mirror carp snout and caudal fin tissues and the isolation of Koi herpesvirus
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The primary culture of mirror carp snout and caudal fin tissues and the isolation of Koi herpesvirus

机译：镜鲤口鼻部和尾鳍组织的原代培养及锦鲤疱疹病毒的分离

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摘要

The explosive Koi herpesvirus (KHV) epidemic has caused the deaths of a large number of carp and carp variants and has produced serious economic losses. The mirror carp (Cyprinus carpio var. specularis) exhibits strong environmental adaptability and its primary cells can be used to isolate KHV. This study utilized the tissue explant method to systematically investigate primary cell culture conditions for mirror carp snout and caudal fin tissues. We demonstrated that cells from these two tissue types had strong adaptability, and when cultured in Medium 199 (M199) containing 20% serum at 26 to 30°C, the cells from the snout and caudal fin tissues exhibited the fastest egress and proliferation. Inoculation of these two cell types with KHV-infected fish kidney tissues produced typical cytopathic effects; additionally, identification by electron microscopy, and PCR indicated that KHV could be isolated from both cell types.
机译：爆炸性的锦鲤疱疹病毒（KHV）流行已导致大量鲤鱼和鲤鱼变种死亡，并造成了严重的经济损失。镜鲤（Cyprinus carpio var。specularis）具有很强的环境适应性，其原代细胞可用于分离KHV。这项研究利用组织外植体方法系统地研究了镜鲤口鼻部和尾鳍组织的原代细胞培养条件。我们证明了来自这两种组织类型的细胞具有很强的适应性，并且当在26至30°C下于含有20％血清的199培养基（M199）中培养时，来自鼻子和尾鳍组织的细胞表现出最快的扩散和增殖。用KHV感染的鱼肾组织接种这两种细胞会产生典型的细胞病变作用。此外，通过电子显微镜和PCR鉴定表明，可以从两种细胞类型中分离出KHV。

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    Jingxiang Zhou; Hao Wang; Xia Zhu; Xingwei Li; Wenliang Lv; Dongming Zhang;

  • 作者单位

    College of Animal Science and Technology Jilin Agricultural University Key Laboratory of Animal Production and Safety of Ministry of Education">(1);

    College of Animal Science and Technology Jilin Agricultural University Key Laboratory of Animal Production and Safety of Ministry of Education">(1);

    College of Animal Science and Technology Jilin Agricultural University Key Laboratory of Animal Production and Safety of Ministry of Education">(1);

    College of Animal Science and Technology Jilin Agricultural University Key Laboratory of Animal Production and Safety of Ministry of Education">(1);

    College of Animal Science and Technology Jilin Agricultural University Key Laboratory of Animal Production and Safety of Ministry of Education">(1);

    College of Animal Science and Technology Jilin Agricultural University Key Laboratory of Animal Production and Safety of Ministry of Education">(1);

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  • 正文语种 eng
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  • 关键词

    Mirror carp; Snout and caudal fin tissue; Primary culture; Koi herpesvirus;

    机译：镜鲤;鼻子和尾鳍组织;初级文化;锦鲤疱疹病毒;

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