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首页> 外文期刊>Waste Management >Biohydrogen and polyhydroxyalkanoates (PHA) as products of a two-steps bioprocess from deproteinized dairy wastes
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Biohydrogen and polyhydroxyalkanoates (PHA) as products of a two-steps bioprocess from deproteinized dairy wastes

机译：生物氢和聚羟基烷烃（PHA）作为脱蛋白乳制品废弃物的双步生物处理的产物

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摘要

In this study a two-steps bioprocess approach aimed at biohydrogen production via dark-fermentation, and polyhydroxyalkanoates-PHA production by mixed microbial cultures, was proposed to valorise two dairy-waste streams coming from cheese whey deproteinization (i.e. Ricotta cheese production and ultrafiltration). During the first step, the increase of OLR was tested, resulting in higher daily H_2 volume (3.47 and 5.07 NL H_2 d~(-1) for second cheese whey-SCW and concentrated cheese whey permeate-CCWP) and organic acids production (14.6 and 12.6 g L~(-1) d~(-1) for SCW and CCWP) for both the substrates, keeping good conversion of sugars into H_2 (1.37 and 1.93 mol H_2 mol~(-1) sugars for SCW and CCWP). During the second step, the organic acids were used for PHA production reaching high conversion yields for both the fermented streams (as average 0.74 ± 0.14 mg COD_(PHA) mg~(-1) COD_(OA-in)), with a maximum polymer content of 62 ±4.5 and 55.1 ± 1.3% (g PHA g~(-1) VSS) for fermented SCW and fermented CCWP respectively. For the results reported, this study could be taken into consideration for larger scale application.
机译：在这项研究中，提出了一种通过暗发酵的两步生物过程方法，并通过混合微生物培养物，对奶酪乳清脱蛋白（即乳清干酪生产和超滤）的两条乳制品废物（即， 。在第一步中，测试了OLR的增加，导致每日更高的H_2体积（3.47和5.07 NL H_2 D〜（-1）用于第二奶酪乳清 - SCW和浓缩奶酪乳清渗透物-CCWP和有机酸生产（14.6对于SCW和CCWP的12.6g L〜（-1）D〜（-1）用于衬底，使糖的良好转化为SCW和CCWP的H_2（1.37和1.93mol H_2Mol〜（-1）糖） 。在第二步中，有机酸用于PHA产生，达到发酵流的高转化率（平均0.74±0.14mg COD_（PHA）Mg〜（-1）COD_（OA-IN）），最大聚合物含量为62±4.5和55.1±1.3％（G PHA G〜（-1）vss），分别用于发酵和发酵的CCWP。对于报告的结果，可以考虑到更大规模的应用程序。

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