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首页> 外文期刊>Waste Management >Assessment of the use of compost stability as an indicator of alkane and aromatic hydrocarbon degrader abundance in green waste composting materials and finished composts for soil bioremediation application
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Assessment of the use of compost stability as an indicator of alkane and aromatic hydrocarbon degrader abundance in green waste composting materials and finished composts for soil bioremediation application

机译：评估堆肥稳定性用作绿色废物堆肥材料中烷烃和芳烃降解的指标和土壤生物化修复应用的成品堆肥

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摘要

Green waste composting materials and finished composts were collected from different commercial ex situ composting sites all treating source segregated green waste feedstocks. Stability of each material was determined using the standard ORG0020 dynamic respiration test. To assess whether stability could be used as an indicator for the potential suitability of green waste composting materials and finished composts as amendments for soil bioremediation, comparison was made with alkane and aromatic hydrocarbon degrader abundance determined using a quantitative PCR (qPCR) approach. Specifically, primers targeting alkB and, polyaromatic hydrocarbon ring-hydroxylating dioxygenases genes (PAH-RHD) of Gram positive (GP) and Gram negative (GN) populations were used for qPCR analysis. The results showed no direct correction between compost stability and gene abundance. Further, increase in alkB gene abundance was not linked to PAH-RHD gene abundance. The results support the use of qPCR as a tool for screening organic amendments on a site by site basis for soil bioremediation treatment.
机译：从不同的商业出原地堆肥网站收集绿色废物堆肥材料和成品堆肥所有处理源隔离绿色废物原料。使用标准ORG0020动态呼吸试验测定每种材料的稳定性。为了评估稳定性是否可以用作绿色废物堆肥材料的潜在适用性和成品堆肥作为土壤生物修复的修正，用使用定量PCR（QPCR）方法测定的烷烃和芳烃降解的比较。具体地，靶向烷烃的引物和克染色（GP）和克克阴性（GN）群的聚芳族烃环 - 羟基羟基酶基因（PAH-RHD）用于QPCR分析。结果表明，堆肥稳定性和基因丰富之间没有直接校正。此外，ALKB基因丰度的增加与PAH-RHD基因丰富无关。结果支持使用QPCR作为筛选遗址筛选有机修正的工具，以通过现场进行土壤生物化治疗。

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  • 来源
    《Waste Management》 |2019年第7期|365-369|共5页
  • 作者

    Diana Guillen Ferrari; Jennifer Pratscher; Thomas J. Aspray;

  • 作者单位

    School of Energy Geoscience Infrastructure and Society Heriot-Watt University Edinburgh EH14 4AS Scotland UK;

    School of Energy Geoscience Infrastructure and Society Heriot-Watt University Edinburgh EH14 4AS Scotland UK;

    School of Energy Geoscience Infrastructure and Society Heriot-Watt University Edinburgh EH14 4AS Scotland UK Solidsense Ltd Bearsden East Dunbartonshire G61 3BA Scotland UK Environmental Reclamation Services Ltd Westerhill Road Bishopbriggs Glasgow G64 2QH Scotland UK;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Compost; Stability; Bioremediation; Alkane; Total petroleum hydrocarbons;

    机译：堆肥;稳定;生物修复;烷烷;总石油碳氢化合物;

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