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首页> 外文期刊>Water, Air, and Soil Pollution >DISCHARGES AND SEDIMENTS AS SOURCES OF EXTRACELLULAR ALKALINE PHOSPHATASE IN A SHALLOW CHINESE EUTROPHIC LAKE
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DISCHARGES AND SEDIMENTS AS SOURCES OF EXTRACELLULAR ALKALINE PHOSPHATASE IN A SHALLOW CHINESE EUTROPHIC LAKE

机译：浅层中国富营养化湖泊中胞外碱性磷酸酶的排放和沉积物

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摘要

Total alkaline phosphatase activity (APA) and soluble reactive phosphorus (SRP) concentrations were measured in municipal wastewater, and a shallow Chinese freshwater lake receiving it. Activities of Dissolved alkaline phosphatase (ADAP) in overlying and interstitial water were also analyzed monthly at three sites for several years. The lake was enriched with SRP and alkaline phosphatase by discharge of the wastewater, indicating that the inclusion of APA for estimating water pollution was reasonable. Annual data showed that APA in coarser fraction was significantly higher at the site receiving more wastewaters, both in surface and overlying water, suggesting that resuspension of enzyme most likely occurred in the basin heavily discharged. ADAP was an order of magnitude higher in the wastewater than those in lake waters, and was generally higher in interstitial water, a feature more striking at the site receiving more discharges. Besides, it was irrespectively inhibited by Na_2WO_4, L-cysteine and EDTA-Na, but stimulated by Cu~(2+), Zn~(2+), CTAB and Triton X-100 in interstitial, overlying and surface waters. This similarity of responding patterns to the stressors indicated an analogy between dissolved alkaline phosphatase in water column and that in interstitial water, supporting the hypothesis that the polluted sediments act as source of dissolved alkaline phosphatase in eutrophic lakes.
机译：测量了市政废水中的总碱性磷酸酶活性（APA）和可溶性反应性磷（SRP）的浓度，并测量了一个浅水湖泊。几年来，还每月在三个站点上分析上覆和间质水中的可溶性碱性磷酸酶（ADAP）的活性。通过排放废水，该湖富含SRP和碱性磷酸酶，这表明将APA纳入估算水污染是合理的。年度数据显示，在地表水和上覆水中接受更多废水的地点，APA中的粗级分明显更高，这表明酶的重悬极有可能发生在大量排放的盆地中。废水中的ADAP比湖水中的ADAP高一个数量级，而间隙水中的ADAP通常更高，此特征在接收更多排放的站点上更为明显。此外，在间隙，上覆和表层水体中，Na_2WO_4，L-半胱氨酸和EDTA-Na均不受抑制，而Cu〜（2 +），Zn〜（2 +），CTAB和Triton X-100刺激。应激源响应模式的这种相似性表明，水柱中的溶解性碱性磷酸酶与间质水中的溶解性碱性磷酸酶之间存在类比，支持以下假设：受污染的沉积物是富营养化湖泊中溶解性碱性磷酸酶的来源。

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