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首页> 外文期刊>Water, Air, and Soil Pollution >PRESENCE OF HEPATITIS A VIRUS IN WATER AND ITS RELATIONSHIP WITH INDICATORS OF FECAL CONTAMINATION
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PRESENCE OF HEPATITIS A VIRUS IN WATER AND ITS RELATIONSHIP WITH INDICATORS OF FECAL CONTAMINATION

机译：水中肝炎病毒的存在及其与粪便污染指标的关系

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摘要

The presence of the hepatitis A virus was studied in spring and river water samples, and heterotrophic plate count, total and fecal coliforms, fecal streptococci, and sulfite-reducing Clostrid-ium were analyzed, in addition to physical-chemical indicators of fecal contamination (conductivity, chemical oxygen demand, nitrate, nitrite, ammonia, phosphate and soluble proteins). The virus was concentrated by means of the aluminium hydroxide adsorption-precipitation method. Following the extraction of RNA, reverse transcriptase PCR, semi-nested PCR and hybridization were performed. No hepatitis A virus was detected in any of the spring water samples, yet 69.6% of the river water samples were positive. There were no significant differences in the mean values of the microbiological indicators when the positive and the negative river samples were compared, which confirms that there is no quantitative relationship between microbiological indicators of fecal contamination and the presence of hepatitis A virus. This was confirmed through a spatial analysis of the river water samples: the point of wastewater discharge was reflected in a high percentage of hepatitis A virus positive samples, regardless of the microbiological indicators. Among the physical-chemical indicators, only conductivity and chemical oxygen demand were significantly correlated with all microbiological indicators. Nitrate and nitrite show limited value as indicators of fecal contamination, and ammonia, phosphate and soluble proteins correlated best with heterotrophic plate count, total coliforms, fecal coliforms and fecal streptococci, which are microbiological indicators of short-term fecal pollution.
机译：在春季和河水样本中研究了甲型肝炎病毒的存在，并分析了粪便污染计数，总粪便和粪便大肠菌群，粪便链球菌和减少亚硫酸盐的梭状芽孢杆菌，以及粪便污染的物理化学指标（电导率，化学需氧量，硝酸盐，亚硝酸盐，氨，磷酸盐和可溶性蛋白质）。通过氢氧化铝吸附沉淀法浓缩病毒。提取RNA后，进行逆转录酶PCR，半巢式PCR和杂交。在任何泉水样品中均未检测到甲型肝炎病毒，但河水样品中有69.6％呈阳性。比较阳性和阴性河流样品时，微生物指标的平均值无显着差异，这证明粪便污染的微生物指标与甲型肝炎病毒的存在之间没有定量关系。这是通过对河水样品进行空间分析得到证实的：无论微生物指标如何，高比例的甲型肝炎病毒阳性样品都反映了废水排放点。在物理化学指标中，只有电导率和化学需氧量与所有微生物指标显着相关。硝酸盐和亚硝酸盐显示为粪便污染指标的价值有限，氨，磷酸盐和可溶性蛋白质与异养菌板数，总大肠菌群，粪便大肠菌群和粪便链球菌的相关性最好，它们是短期粪便污染的微生物指标。

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