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Effect of mixing intensity on hydrolysis and acidification of sewage sludge in two-stage anaerobic digestion: Characteristics of dissolved organic matter and the key microorganisms

机译:二级厌氧消化过程中混合强度对污泥水解酸化的影响:溶解性有机物和关键微生物的特征

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Mixing should be optimized in anaerobic digestion (AD) systems to achieve excellent biomaterials production in the sewage sludge (SS) management in wastewater treatment plant. AD depends on the coordinated activity of hydrolysis, acidification and methanogenesis. However, the effect of mixing intensity on characteristics of hydrolysis and acidification in AD of SS is still poorly understood. This study focused on the mixing intensity (30, 60, 90 and 120 rpm) effect on the characteristics of dissolved organic matter (DOM) and the key microorganisms in the hydrolysis and acidification of SS. Results showed that enhanced hydrolysis and acidification efficiency was obtained at mixing of 90 and 120 rpm (p 0.05), while the maximum acetic acid (388 +/- 21 mg/L) was produced at 90 rpm. Mixing at 90 rpm enhanced the release of protein and polysaccharide as well as humic acid. Further analyses of DOM molecular features revealed that 90 rpm led to the highest molecular diversity and easily biodegradable molecules (lipid and proteins/amino sugars), which contributed to the maximum hydrolysis and acidification efficiency. Firmicutes and Actinobacteria significantly increased with mixing intensity (p0.05), and Chloroflexi and Fusobacteria were enriched at mixing of 90 rpm, which favored the hydrolysis of SS. The enrichment of Clostridium XI and Clostridium sensu strict contributed to the acidification of DOM at 90 and 120 rpm. The results of this study can advance our knowledge about mixing intensity effects on the AD systems of SS. This research also showed how increasing mixing intensity to a relatively high speed can enhance the hydrolysis and acidification efficiency of SS. (C) 2018 Elsevier Ltd. All rights reserved.
机译:应在厌氧消化(AD)系统中优化混合,以在废水处理厂的污水污泥(SS)管理中实现出色的生物材料生产。 AD取决于水解,酸化和甲烷生成的协调活性。然而,对于SS的AD中混合强度对水解和酸化特性的影响仍然知之甚少。这项研究集中于混合强度(30、60、90和120 rpm)对SS水解和酸化过程中溶解性有机物(DOM)和关键微生物特性的影响。结果表明,在90和120 rpm混合时,水解和酸化效率提高(p <0.05),而在90 rpm时产生最大乙酸(388 +/- 21 mg / L)。以90 rpm的速度混合可增强蛋白质和多糖以及腐殖酸的释放。对DOM分子特征的进一步分析表明,以90 rpm的转速可获得最高的分子多样性和易于生物降解的分子(脂质和蛋白质/氨基糖),从而有助于最大程度地水解和酸化。硬菌和放线菌随着混合强度的增加而显着增加(p <0.05),并且在90 rpm的混合下,绿弯曲菌和融合细菌富集,有利于SS的水解。梭状芽胞杆菌XI和梭状芽孢杆菌的富集严格促进了90和120 rpm时DOM的酸化。这项研究的结果可以提高我们有关混合强度对SS AD系统的影响的知识。这项研究还表明,将混合强度提高到较高的速度可以如何增强SS的水解和酸化效率。 (C)2018 Elsevier Ltd.保留所有权利。

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