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首页> 外文期刊>Water Research >Monitoring associations between clade-level variation, overall community structure and ecosystem function in enhanced biological phosphorus removal (EBPR) systems using terminal-restriction fragment length polymorphism (T-RFLP)
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Monitoring associations between clade-level variation, overall community structure and ecosystem function in enhanced biological phosphorus removal (EBPR) systems using terminal-restriction fragment length polymorphism (T-RFLP)

机译：使用末端限制片段长度多态性（T-RFLP）监测增强型生物除磷（EBPR）系统中进化枝水平变异，总体群落结构和生态系统功能之间的关联

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The role of Candidatus "Accumulibacter phosphatis" (Accumulibacter) in enhanced biological phosphorus removal (EBPR) is well established but the relevance of different Accumulibacter clades to the performance of EBPR systems is unknown. We developed a terminal-restriction fragment length polymorphism (T-RFLP) technique to monitor changes in the relative abundance of key members of the bacterial community, including Accumulibacter clades, in four replicate mini-sequencing batch reactors (mSBRs) operated for EBPR over a 35-day period. The ability of the T-RFLP technique to detect trends was confirmed using fluorescence in situ hybridisation (FISH). EBPR performance varied between reactors and over time; by day 35, performance was maintained in mSBR2 whilst it had deteriorated in mSBRl. However, reproducible trends in structure-function relationships were detected in the mSBRs. EBPR performance was strongly associated with the relative abundance of total Accumulibacter. A shift in the ratio of the dominant Accumulibacter clades was also detected, with Type IA associated with good EBPR performance and Type IIC associated with poor EBPR performance. Changes in ecosystem function of the mSBRs in the early stages of the experiment were more closely associated with changes in the abundance of (unknown) members of the flanking community than of either Accumulibacter or Candidatus "Competibacter phosphatis". This study therefore reveals a hitherto unrecorded and complex relationship between Accumulibacter clades, the flanking community and ecosystem function of laboratory-scale EBPR systems.
机译：念珠菌“ Accumulibacter phosphatis”（Accumulibacter）在增强生物除磷（EBPR）中的作用已被充分确立，但是不同的Accumulibacter进化枝对EBPR系统性能的相关性尚不清楚。我们开发了一种末端限制性片段长度多态性（T-RFLP）技术，用于在四个重复的小序列分批反应器（mSBR）中监测细菌群落关键成员的相对丰度变化，包括Accumulibacter进化枝。 35天期限。使用荧光原位杂交（FISH）证实了T-RFLP技术检测趋势的能力。 EBPR性能在反应堆之间以及随时间变化；到第35天，mSBR2的性能得以维持，而mSBR1的性能却有所下降。但是，在mSBR中检测到结构-功能关系的可重复趋势。 EBPR性能与总积累细菌的相对丰度密切相关。还检测到占优势的Accumulibacter进化枝比例发生了变化，其中IA型与良好的EBPR性能相关，IIC型与不良的EBPR性能相关。在实验的早期阶段，mSBRs的生态系统功能的变化与侧翼社区（未知）成员的丰度变化相比更紧密相关，而不是Accubaculibacter或Candidatus“ phosphetatibacter phosphatis”。因此，这项研究揭示了迄今未记录的，实验室规模的EBPR系统的Accumulibacter进化枝，侧翼群落和生态系统功能之间的复杂关系。

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