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首页> 外文期刊>Water Research >Reactivation and growth of non-culturable indicator bacteria in anaerobically digested biosolids after centrifuge dewatering
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Reactivation and growth of non-culturable indicator bacteria in anaerobically digested biosolids after centrifuge dewatering

机译：离心脱水后厌氧消化的生物固体中不可培养指示菌的活化和生长

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摘要

Recent literature has reported that high concentrations of indicator bacteria such as fecal coliforms (FCs) were measured in anaerobically digested sludges immediately after dewatering even though low concentrations were measured prior to dewatering. This research hypothesized that the indicator bacteria can enter a non-culturable state during digestion, and are reactivated during centrifuge dewatering. Reactivation is defined as restoration of culturability. To examine this hypothesis, a quantitative polymerase chain reaction (qPCR) method was developed to enumerate Escherichia coli, a member of the FC group, during different phases of digestion and dewatering. For thermophilic digestion, the density of E. coli measured by qPCR could be five orders of magnitude greater than the density measured by standard culturing methods (SCMs), which is indicative of non-culturable bacteria. For mesophilic digestion, qPCR enumerated up to about one order of magnitude more E. coli than the SCMs. After centrifuge dewatering, the non-culturable organisms could be reactivated such that they are enumerated by SCMs, and the conditions in the cake allowed rapid growth of FCs and E. coli during cake storage.
机译：最近的文献报道，脱水后立即在厌氧消化的污泥中测定了高浓度的指示菌，例如粪便大肠菌（FCs），即使在脱水之前测得的浓度低。这项研究假设指示剂细菌可以在消化过程中进入不可培养状态，并在离心脱水过程中被重新活化。重新激活被定义为可培养性的恢复。为了检验该假设，开发了一种定量聚合酶链反应（qPCR）方法来枚举在消化和脱水的不同阶段中FC组成员的大肠杆菌。对于嗜热消化，通过qPCR测量的大肠杆菌密度可能比通过标准培养方法（SCM）测量的密度高五个数量级，这表明细菌不可培养。对于中温消化，qPCR所列举的大肠杆菌比SCM多约一个数量级。离心脱水后，不可培养的生物可以被重新激活，从而被SCM枚举，并且滤饼中的条件允许滤饼存储过程中FC和大肠杆菌的快速生长。

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