...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Weed Science >Biological Control Agent for Rice Weeds from Protoplast Fusion between Curvularia Lunata and Helminthosporium Gramineum
【24h】

Biological Control Agent for Rice Weeds from Protoplast Fusion between Curvularia Lunata and Helminthosporium Gramineum

机译：弯孢菌和禾本科毛孢菌原生质体融合的稻草生物防治剂

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The advancement of biological weed control is limited by the slow development of effective, broader-spectrum biological control agents. Protoplast fusion was carried out between the Helminthosporium gramineum subsp. echinochloae (HGE) strain, HM1, and Curvularia lunata (CL) to breed new strains with improved biocontrol efficiency. The HM1 strain was derived from HGE by ultraviolet (UV) treatment. Conditions for protoplast fusion were optimized, including lytic enzyme mixtures and incubation time. The efficacy of lytic enzyme mixtures on cell wall digestion was also compared. The most effective lytic enzyme mixture for CL was 2% lywallzyme plus 2% snailase and for HM1, 2% cellulase plus 2% snailase. The optimum incubation time was 16 h for CL and 24 h for HM1. All fusant strains exhibited similar morphological and conidial properties to the HM1 parent. A total of 1,360 fusant strains were produced, 136 of which were randomly selected for characterization. Seven fusant strains showed improved spore productivity and four fusant strains had increased production of the phytotoxin ophiobolin A, compared with HM1. Random amplified polymorphic DNA (RAPD) analysis of the fusant strains showed that the seven phytoactive fusant strains had a high similarity index (95 to 99%) to the parent HM1, but low similarity (22 to 26%) to the parent CL. Ethyl acetate extract of the culture broth and mycelia effectively controlled major weeds in rice production.
机译：有效的，广谱的生物防治剂的缓慢发展限制了生物除草的进展。原生质体融合在禾本科蠕孢亚种之间进行。棘轮虫（HGE）菌株HM1和弯孢弯曲菌（CL。Curvularia lunata（CL））来育种具有更高生物防治效率的新菌株。 HM1菌株是通过紫外线（UV）处理从HGE衍生而来的。优化了原生质体融合的条件，包括裂解酶混合物和孵育时间。还比较了裂解酶混合物对细胞壁消化的功效。对于CL而言，最有效的裂解酶混合物是2％的lywallzyme加2％的Snailase，对于HM1，最有效的裂解酶混合物是2％的纤维素酶加2％的Snailase。 CL的最佳孵育时间为16小时，HM1的最佳孵育时间为24小时。所有融合菌株均表现出与HM1亲本相似的形态和分生孢子特性。总共生产了1360种融合菌株，其中136种是随机选择进行鉴定的。与HM1相比，七个融合菌株显示出提高的孢子生产力，四个融合菌株提高了植物毒素ophiobolin A的产量。对融合菌株进行随机扩增多态DNA（RAPD）分析表明，这七个具有植物活性的融合菌株与亲本HM1具有较高的相似性指数（95％至99％），而与亲本CL的相似性较低（22％至26％）。培养液和菌丝体的乙酸乙酯提取物可有效控制水稻生产中的主要杂草。

著录项

  • 来源
    《Weed Science》 |2007年第6期|p.599-605|共7页
  • 作者

    Z. B. Zhang N. R. Burgos J. P. Zhang and L. Q. Yu*;

  • 作者单位

    * First, third, and fourth authors: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou, China 310006;

    second author: Department of Crop, Soil, and Environmental Sciences, 1366 W. Altheimer Drive;

    University of Arkansas, Fayetteville, AR 72704. Corresponding author's E-mail: liuqyu53@yahoo.com.cn;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biological weed control, efficacy, weeds in rice;

    机译：生物除草;功效;水稻中的杂草;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号