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Evaluating Seed Viability by an Unimbibed Seed Crush Test in Comparison with the Tetrazolium Test

机译:与四唑试验比较,通过整体种子粉碎试验评估种子生存力

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摘要

The unimbibed crush test, in which seed viability is evaluated by crushing and visual inspection of dry seeds, was compared with tetrazolium staining, an established method of testing seed viability. The unimbibed crush test potentially provides an immediate and rapid method for determining seed viability. Six sets of seed lots, involving the four weed species, velvetleaf, common lambsquarters, redroot pigweed, and smooth pigweed, were tested by each method. For four of the six sets, results from the crush test were statistically indistinguishable from those of the tetrazolium test. For the other two sets of seed lots, the crush test indicated higher viability than did the tetrazolium test. The crush test may be most useful for seed bank surveys in which many samples are typically processed, and most of the variation in density of viable seeds is associated with number of seeds present rather than percentage viability. Its use in more exacting circumstances like seed survival studies requires caution.
机译:通过粉碎和目测检查干燥种子来评估种子生存力的无吸收压榨试验与四唑鎓染色进行了比较,四唑染色是一种确定的种子生存力测试方法。未经吸收的压碎测试可能会提供一种直接,快速的方法来确定种子的活力。每种方法测试了六组种子,包括四种杂草,绒毛,普通羊羔,红根杂草和光滑杂草。对于六组中的四组,压碎试验的结果与四唑鎓试验的结果在统计学上没有区别。对于其他两组种子批次,压碎试验显示比四唑试验更高的生存力。压碎测试对于种子库调查可能是最有用的,在种子库调查中通常要处理许多样品,并且有活力种子的密度变化中的大多数与存在的种子数量而不是生存力百分比相关。在更严格的情况下(例如种子存活研究)使用它需要谨慎。

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