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首页> 外文期刊>World Journal of Microbiology and Biotechnology >Cometabolic degradation of mono-chloro benzoic acids by Rhodococcus sp. R04 grown on organic carbon sources
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Cometabolic degradation of mono-chloro benzoic acids by Rhodococcus sp. R04 grown on organic carbon sources

机译：红球菌的新陈代谢降解一氯苯甲酸。 R04生长在有机碳源上

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摘要

The aerobic cometabolism of chlorobenzoic acids (CBAs) by Rhodococcus sp. R04 was accomplished by augmenting the medium with organic carbon sources. In mineral medium supplemented with glucose (MMG), 0.5 mM 2-CBA was incompletely metabolized after the 5-day incubation, while the near-complete disappearance of 0.5 mM 4-CBA was monitored. Over the 5-day incubation period, the concentration of chloride increased to 0.17 mM in bottles containing 4-CBA, glucose and strain R04; whereas in cultivation with 2-CBA the chloride content was about 0.1 mM. After 5-day incubation, 28.5% 4-CBA was remained in mineral medium supplemented with ethanol (MME), and the relatively low values of chloride were released. To our knowledge, it is first report that the feasibility of using ethanol as an added substrate for cometabolic degradation of CBA by aerobic polychlorinated biphenyl (PCB)-degrading bacteria. The specific activities of (chloro)benzoate 1,2-dioxygenase and (chloro)catechol 1,2-dioxygenase activities were detected in cell-free extracts (CFEs) of strain R04. These results suggest that the initial degradation of CBAs occurred most likely prior to chloride release.
机译：Rhodococcus sp。的需氧代谢氯苯甲酸（CBAs）。通过用有机碳源增加培养基来实现R04。在培养5天后，在补充了葡萄糖（MMG）的矿物质培养基中，0.5 mM 2-CBA不完全代谢，同时监测了0.5 mM 4-CBA的接近完全消失。在5天的温育期中，装有4-CBA，葡萄糖和R04菌株的瓶子中的氯化物浓度增加到0.17 mM。而在使用2-CBA进行培养时，氯化物含量约为0.1 mM。孵育5天后，在添加了乙醇（MME）的矿物培养基中保留了28.5％的4-CBA，并释放出相对较低的氯化物。据我们所知，第一个报道是使用乙醇作为有氧多氯联苯（PCB）降解细菌可代谢降解CBA的底物的可行性。在菌株R04的无细胞提取物（CFE）中检测到（氯）苯甲酸酯1,2-二加氧酶和（氯）邻苯二酚1,2-二加氧酶的比活性。这些结果表明，CBA的初始降解最有可能发生在氯化物释放之前。

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  • 来源
    《World Journal of Microbiology and Biotechnology》 |2009年第7期|1169-1174|共6页
  • 作者

    Guoqing Zhang; Xiuqing Yang; Fuhong Xie; Yapeng Chao; Shijun Qian;

  • 作者单位

    State Key Laboratories of Transducer Technology Institute of Microbiology Chinese Academy of Sciences 100101 Beijing China;

    Institute of Biotechnology Shanxi University Taiyuan China;

    State Key Laboratories of Transducer Technology Institute of Microbiology Chinese Academy of Sciences 100101 Beijing China;

    State Key Laboratories of Transducer Technology Institute of Microbiology Chinese Academy of Sciences 100101 Beijing China;

    State Key Laboratories of Transducer Technology Institute of Microbiology Chinese Academy of Sciences 100101 Beijing China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Cometabolic degradation; CBA; Dechlorination; Glucose; Ethanol;

    机译：代谢分解;CBA;脱氯;葡萄糖;乙醇;

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